Hypoxia Preconditioning HK-Ⅱ Regulation In MPTP Relieve Cardiomyocytes Hypoxia/reoxygenation Injury

Objective: To explore the mechanism of myocardial protective effect of hypoxic preconditioning(HPC) play by HIF-1α regulates the HK-2 reduce mPTP open. Methods: In the model of hypoxia/reoxygenation(H/R) of cultured neonatal rat cardiomyocytes, After 72 h divided into 6 groups:(1) control group(CON),(2) Hypoxia Group(H/R),(3) hypoxic preconditioning(HPC),(4) the control +HIF-1 Inhibitor Group(CON+YC-1),(5) Hypoxic preconditioning + HIF-1 Inhibitor Group(HPC+YC-1),(6)Dimethyl sulfoxide Group(DMSO).After reoxygenation test myocardial cell apoptosis, HIF-1α, HK-Ⅱ, Cyt C protein and mitochondrial membrane potential. Results: HPC,HPC+YC-1 rate of apoptosis, Cyt c protein content less then H/R have statistical significance(p<0.05), and HPC+YC-1 is higher than the HPC have statistical significance(p <0.05). HPC,HPC+YC-1 of mitochondrial membrane potential,HIF-1α, and HK-Ⅱ protein content, above H/R have statistical significance( P < 0.05), HPC+YC-1 below the HPC have statistical significance( P < 0.05)Conclusion: HPC have protective effects on neonatal rat cardiomyocytes against lethal H/R injury, involved in the mechanism of HIF-1α regulates the HK-Ⅱ reduce mPTP open, reducing myocardial damage. Show stability of HIF-1α, to increase HK-Ⅱ to enhance the effect of HPC on myocardial protection, which provides a potential target for clinic.