The Protective Effect Mechanisms About Ascorbic Acid On Traumatic Brain Injury In Rats

Objective Investigate the protective effect of ascorbic acid on secondary brain injury caused by traumatic brain injury and its possible mechanism.Methods The rats were randomly divided into sham + saline group, sham + ascorbic acid group,hit + saline group and hit + ascorbic acid groups. Establish moderate traumatic brain injury model,24 hours after traumatic brain injuries neurological injury score; 24 hours after traumatic brain injury brain water content was measured; measured 24 hours after traumatic brain barrier permeability; The activity of superoxide dismutase in brain tissue were measured by ELISA;by Western blot After determination of traumatic brain injury 24 hours of brain tissue damage tumor necrosis factor-α(TNF- α), CYP2E1,Occludin, MMP-9(matrix metalloprotein-9) and aquaporin 4(AQP-4) expression.Results hit + saline group, the degree of neurologic injury blow + ascorbic acid group mitigate and reduce brain edema, significantly reduced the extent of damage to the blood brain barrier, TNF-α, CYP2E1, MMP-9 and AQP-4 reduced, The activity of superoxide and Occludin increased.Conclusion ascorbic acid can reduce brain edema and fight against the destruction of the blood-brain barrier.The mechanism is inhibiting CYP2E1’s expression.