| Background:Cerebral ischemic disease is a common and frequently occurring disease in clinic. Its morbidity and mortality are high, and its pathogenesis is complex. There are many ways of secondary damage. The inflammatory reaction is one of the important reasons causing the injury of cerebral ischemia reperfusion injury. How to reveal the key links and reasonable intervention to reduce cerebral ischemia reperfusion injury is a hot research topic at present, and also the starting point of this research.HMGB1 is a kind of important "late" inflammatory cytokines, which may be a central link in the regulatory network of inflammatory cytokines, HMGB1 release is increased and the release of HMGB1 into the blood is further expanded in the form of endocrine and para. As an important "late" inflammatory cytokines, HMGB1 can not only induce the expression of TNF-, IL-1, but also activate ERK kinase, K P38MAP kinase, JNK kinase and induce the nuclear translocation of nuclear transcription factor NF-kappa B. These studies suggest that HMGB1 may be a central link in the regulation of inflammatory cytokines, not only the biological effect of its own secretion, but also to regulate the secretion of other inflammatory factors. A large number of experiments have proved that HMGB1 is closely related with the heart, spinal cord and intestinal tract and other organs, but it is also important to play an important role in cerebral ischemia reperfusion injury.L-carnitine, also known as L-carnitine, substance and energy metabolism, a large number of experiments confirmed L-carnitine beta oxidation, can satisfy the cerebral ischemia and reperfusion injury of ATP energy demand. And can enhance pyruvate dehydrogenase activity, reduce the formation of cerebral lactate, increase the rate of glucose utilization and on cerebral ischemia and reperfusion injury to the protective effect. We also of L-carnitine on cerebral ischemia reperfusion injury were studied and investigate its and inflammatory cytokines, HMGB1, further study the cerebral protective effects of whether and inflammatory cytokines, HMGB1 related.Objective:1:To establish the rat cerebral ischemia reperfusion model.2:To observe the changes of brain tissue in rats with cerebral ischemia and reperfusion in rats.3:To observe the apoptosis and the expression of HMGB1 in rats with cerebral ischemia and reperfusion4:To study the effect of L-carnitine on experimental ischemia reperfusion rats.Methods:Experimental groups:45 rats were divided to 3 groups, sham operation group (sham group), the rats with ischemia reperfusion group (IR group), L-carnitine intervention group (LC), in rats with cerebral ischemia again perfusion money continuous levocarnitine injection 200mg/kgl week, through the rat right middle cerebral artery occlusion of preparation of cerebral ischemia reperfusion model. After modeling,24h and brain tissue samples were collected.Results:1:TTC staining method, SHAM group and LC group in the area of cerebral infarction were increased compared with IR group, suggesting that the brain injury of ischemia reperfusion injury. The volume of cerebral infarction in LC group was significantly lower than that in IR group (P<0.05).2:The apoptosis index of IR group was significantly higher than that of SHAM group, while the apoptosis index of LC group was lower than that of CR group (P<0.05).3:ELISA assay showed that the expression of MDA and malondialdehyde (HMGB1) were significantly increased after cerebral ischemia and reperfusion, and the expression of superoxide dismutase (SOD) was decreased, and HMGB1 and MDA in LC group were significantly lower than that in IR group (#P<0.05), and the expression of SOD was higher than that in IR group.4:WB test results indicated that the expression of HMGB1 protein in brain tissue increased after cerebral ischemia reperfusion (**P<0.01), while the HMGB1 protein in LC group was significantly lower than that in IR group (#P<0.05).Conclusions:1:HMGB1 plays an important role in cerebral ischemia reperfusion injury in rats;2:L-carnitine on rat cerebral ischemia reperfusion injury has a protective effect;3:the brain protection mechanism of L-carnitine may be related to the inhibition of HMGB1 expression. |
PDF Full Text Request