Protective Effect Of Procyanidins On Cisplatin-induced Hair Cell Damage In HEI-OC1 Auditory Cells

Objective To evaluate the protective effects and mechanism of procyanidins (OPC) on cisplatin-induced damage in HEI-OC1 auditory cell lines.Methods Cell viability was measured by MTT assay and microscopic observation. Apoptosis was determined by Hoechst 33258 staining using fluorescence microscope.lntracellular reactive oxide species (ROS) were analyzed by flow-cytometry. The mRNA expression of pro-apoptosis factorbax and anti-apoptosis factorbcl-2 were assayed by real-time PCR. The expression levels of cleaved Caspase-3 and Caspase-8 were evaluated by Western blotting. Results Compared with cisplatin injury group, OPC increased cell viability as demonstrated by the MTT assay and microscopic observation; OPC decreased the cell apoptosis by Hoechst 33258 staining(p<0.05); OPC decreased intracellular ROS production (p<0.01); OPC decreased the expression of bax mRNA and increased the expression of bcl-2 mRNA (p<0.05); OPC decreased the expression of cleaved Caspase-3 and Caspase-8 (p<0.05). Conclusions OPC attenuated cisplatin-induced HEI-OC1 apoptosis, these effects were mediated by its radical scavenging activity and inhibition of apoptosis.