| Objective:Endometriosis (EMs), referred to as endometriosis, is a common disease in women of childbearing age, the incidence rate of 10%-15%, and had the obvious rise trend. The pathogenesis of endometriosis, extensive disease complex, diverse, and the biological behavior of malignant and invasive growth, metastasis and recurrence of widely cultivated, is a common and difficult to treat gynecological diseases. At present, endometriosis treated mainly by surgical castration operation and hormone therapy, but the effect is limited, side effects, easy recrudesce, incurable, has become one of the difficult problems in gynecology and obstetrics. So far the endometriosis pathogenesis is still not clear, in many theories, endometrial implantation theory proposed by Sampson in 1925 is one of the main theory is popular, but endometrial planting and its invasion and metastasis is a complex process involving multiple factors. In recent years, about EMs in endometrial itself with the characteristics and its role in the pathogenesis, gradually attracted people’s attention. Nuclear transcription factor Kappa B (nuclear factor-kappa B. NF-κB) is a nuclear transcription factor which widely exist in the body cells, is an important transcription factor exists in cells. The general condition is based on the homologous or heterologous two dimeric form and its inhibition of protein I κ B formed a complex, non active state exists in cytoplasm, were activated by harmful factors stimulation, activation of NF-kappa B from NF-κ B-I κ Bs complex dissociation, enter the nucleus, combined with the corresponding target gene, transcription. NF-κ B can efficiently induce proinflammatory cytokines (IL-1 β, TNF-a), expression of leukocyte adhesion molecules, monocyte chemotactic factor gene, but also has an important role in regulating gene transcription of various enzymes involved in the inflammatory cascade and amplification effect, has been paid more and more attention. Neutrophil epithelial activation peptide-78(ENA-78) is one of the important chemokines produced NF-κ BP65 activation following, is one of the important chemokines and angiogenesis factor, the chemotaxis of neutrophils to focus and direct promotion of ectopic endometrial cell proliferation, the IL-1 beta, TNF a (TNF a), inducer, released by macrophages, ectopic endometrial cells to synthesize and. But the regulation of NF-kappa B on ENA-78 in endometriosis is unknown. Interleukin-8 (IL-8) is a kind of inflammatory monocytes-macrophage chemotactic factor generated, but also of angiogenic factors. Chemotaxis of neutrophils and mononuclear cells in the inflammatory response, the activation of inflammatory cells, leading to ectopic endometrial inflammation, the formation of adhesions; at the same time it is angiogenesis factor, can make the ectopic focus of new blood vessel formation, is conducive to the ectopic endometrium successful planting and growth. Studies have shown that activation of NF-kappa Bexpression can be induced by IL-8. Proline two thiocarbamate inhibitor (PDTC) is a NF-kB specificantagonist, scavenging oxygen free radicals, which can effectively inhibit NF-κB activity, reduce the TNF-α, IL-10, IL-8, VEGF, mRNA level makes, thereby blocking occurs through NF-kappa B pathway biolog. By detecting the expression level of IL-8, ENA-78mRNA in the ectopic endometrium and normal endometrium in the rat model of endometriosis, endometrial. serum, explore its relationship with endometriosis, and investigate effects of PDTC on the level of ectopic lesion volume, IL-8, ENA-78 expression in rat model of endometriosis.Methods:1.The establishment of SD rat model of endometriosis:Morphological changes of rat estrous monitoring were observed in 48 SD rats vaginal exfoliated cells, selection of rat with 2 or more consecutive estrous cycle, on third estrus, reference Jones autologous endometrial implantation, for endometrial implant, the establishment of SD rat model of endometriosis. Transplantation showed a clear cyst and cyst containing light yellow transparent cyst fluid accumulation are considered as successful model.2. Volume measurement of ectopic focus model in rats:Modeling the success of 40 rats were randomly divided into 4 groups. A (control group, only normal feeding), B (progesterone group, intraperitoneal injection of 10mg/kg/d), C(PDTC,100mg/kg/d,intraperitoneal injection) and D group (PDTC+ progesterone, the same amount of B, C group); In fourth, measured eighth weeks after modeling the size of the ectopic foci, and calculate volume (volume according to the formula:V=0.52 x length x width x height).3.Detection of serum IL-8 levels in:using enzyme-linked immnunosorbent assay(ELISA) method for the determination of the content of IL-8 in serum of SD rats.4.Detection of the expression level of ENA-78mRNA in rats with lesions: Using reverse transcription polymerase chain reaction (RT-PCR) detection of four groups of rats in the expression levels of ENA-78mRNA endometrium. ectopic endometrium and normal endometriosis.Results:1. To establish the rat model of SD:48 rats model.40modeling success, success rate was 83.33%,5 of them never die.3 rats died of intra-abdominal abscess.2. The growth of ectopic lesions:4weeks after modeling, A, B, C, D four groups in the rat model of different focal volume comparison and found that after drug intervention of B, C, D three groups of rats in different focal volume significantly less than group A, the difference was statistically significant (P< 0.05). Ectopic lesions volume of C, D group was obviously less than group B, the difference was statistically significant (P< 0.05). Volume is less than in group C, group D ectopic lesions differences have statistical significance (P< 0.05). At 8 weeks between groups of ectopic lesions size comparison, the same as 4 weeks. Will be 8 weeks and 4 weeks of four groups of ectopic lesions volume comparison found that group A ectopic lesions are that increases with the extension of time, difference was statistically significant (P< 0.05); After drug intervention of B, C, D three groups of ectopic lesions are reduced gradually with the extension of time, difference have statistical significance (P< 0.05).3. ELISA method to detect after treatment between groups of rats serum IL-8 levels:4 weeks after modeling, the A, B, C, D four groups of rats when comparing the levels of serum I-8 found that drugs after the intervention of B, C, D of three groups of rats serum IL-8 level was lower than that in group A, the difference was statistically significant (P< 0.05). Group C and group D rat serum IL-8 level was lower than that in group B, the difference was statistically significant (P< 0.05). Group D rat serum IL-8 level lower than that of group C, difference have statistical significance (P< 0.05). Thursday group rats serum IL-8 of 8 horizontal comparison, the same as 4 weeks. Will be 8 weeks and 4 weeks of drug intervention of three groups of rats serum IL-8 horizontal comparison, found that the former is lower than the latter, and gradually decreased with longer duration of use, difference was statistically significant (P< 0.05).4. RT-PCR method to detect the rat ENA-78 mRNA expression in ectopic lesions:4 weeks after modeling, B, C, D after drug intervention in the three groups of rats ectopic foci ENA-78 mRNA expression was lower than that in group A, the difference was statistically significant (P< 0.05). C, D group rats ectopic foci in the ENA-78 mRNA expression was significantly lower than group B, difference was statistically significant (P< 0.05); Group D rat ectopic foci in the ENA-78 mRNA expression is lower than in group C, difference have statistical significance (P< 0.05). At 8 weeks four groups of rats with ectopic foci in the ENA-78 mRNA expression, with 4 weeks. Will be 8 weeks and 4 weeks after drug intervention of three groups of rats ENA-78 mRNA expression in ectopic foci found after comparison, the former is lower than the latter, difference was statistically significant (P< 0.05).Conclusion:1.The NF-κ B blocker PDTC treatment of rats in different disease models effectively.2. The PDTC and used in combination with progesterone, the treatment effect is remarkable, that both in the process of treatment in different syndrome may have synergy.3.Different disease of NF-κ B likely to ENA-78 sampling control and other cytokines play a central role. |
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