| 1 BackgroundChronic Rhinosinusitis with Nasal Polyps(CRSwNP) is a highly heterogeneous disease of nasal mucosa which is caused by multiple factors and develops in many steps. It is characterized by single or multiple polyps consist of heavy edema of nasal mucosa. Also, we can see many inflammatory cells in the tissue of nasal polyps,especially for Eosinophils. Nowadays, numerous researches show that play an important role in the formation of nasal polyps which include both changed innate and adaptive immune system, tissue reconstruction, abnormal arachidonic acid metabolism and/or the effect of microbe. However, its definite etiology is still unknown. Autophagy is a progress of keeping stable and maintaining balance for cell under stress, in which the metabolic wastes are removed, cell structures update. So far, Beclinl and P62 are key autophagy related genes, there has been little report about autophagy in nasal polyps.2 ObjectiveIn this study,we detect the expression of Beclinl and P62 in both protein and mRNA level which aims to discuss the expression of autophagy in CRSwNP and its clinical significance.3 MethodsThe specimens were obtained from patients who were treated by endoscopic surgery from October 2014 to April 2015 in department of Otolaryngology in Qilu Hospital, Shandong University. They were divided into two groups:nasal polyp tissue as experimental group (n=50,male 26,female 24,aged 24-71,average 47.1) and normal inferior turbinate mucosa as control group (n=20, male 12,female 8,aged 18-54,average 35.2). The diagnosis was based on CRS guide in Kunming 2012. Inclusion criteria were the patients who took no pills or drugs such as antibiotics, systemic or nasal glucocorticoid and so on. Exclusion criteria were the patients who were suffered from tumor, immunodeficiency and other severe systemic disease at the beginning. At the same time, the cases in control group should not suffer from allergic rhinitis according to allergen skin prick test. Then, CT scans was used to exclude nasal sinuses occupied disease. Severity of illness was evaluated by VAS scores and Lund-Mackay scores in CT scan. This research has been admitted by ethics committee of Qilu Hospital, Shandong University. All of the recruited patients have signed the informed consent. The tissue in general morphology was detected with Hematoxylin-eosin (HE) staining, the number of Eosinophils was calculated in five visual filed under high power lens, then the polyps tissues were divided into ECRSwNP in which the average ratio is higher than 10 percent, another were attributed to non-ECRSwNP which is lower than 10 percent. The expression of Beclinl and p62 was examined with immuno-histochemistry(IHC) and real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR). SPSS 20.0 software was used to analyze the data.4 ResultsExpression of Beclinl and P62 in protein level:the expression of Beclinl in nasal polyp tissue was lower than inferior turbinate mucosa(U value is-13.36, P <0.01),in contrast, P62 in experimental group was higher than control group(U value is 12.99, P<0.01). Both differences were significant in statistics(P<0.05).Expression of Beclinl and P62 in mRNA level:the relative quantity of Beclinl and LC3B expressions in nasal polyp were 0.46±0.17 and 0.46±0.11, which was lower than those in turbinate mucosa 1.11±0.47 and 0.96±0.25. Relative quantity of P62 expression in nasal polyp was 2.19±0.44, which was higher than that in turbinate mucosa 1.05±0.33. The differences were all significant in statistics(P <0.05).5 ConclusionCompared with control group as nasal superior turbinate, the expression of nasal polyp tissue was declined in Beclinl and little highed in P62 which evealed that autophagy was deficient in nasal polyps, and it may be in connection with the pathogenesis of nasal polyps. |
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