| Objectives:To investigate the effects and mechanism of cordycepin on proliferation and migration of gallbladder cancer.Materials and methods:By using MTT assay, gallbladder cancer cell proliferation was assessed after treated with cordycepin. The colony formation of SNU308 cells treated with different concentration of cordycepin was tested by colony formation assay. Wound Healing assay and migration assay were detected cordycepin on the SNU308 effect of cell migration in vitro. The effect of cordycepin-induced apoptosis was detected by flow cytometry. The effect of cordycepin-induced autophagy was examined by immunofluorescence and western blot.Results:1. MTT assay results showed that compare with the control group, cordycepin on 5μM, lOμM,20μM and 30μM inhibited cell proliferation of SNU308 following treated at 24h and 48h, and showed a time-and dose-dependent (p<0.05).2. Results of colony formation assay showed that cordycepin also inhibited the colony formation following treated with 10μM and 20μM cordycepin at 7 days (p<0.05).3. Results of flow cytometry showed that cell apoptosis significantly induced when treated with 20μM cordycepin.4. Western blot analysis showed that cordycepin enhanced the expression of Beclin-1 and Bax, and regulated Bcl-2 expression. In addition, immunofluorescence results showed that the expression and intensity of LC3 protein obviously increased following treated with 20μM cordycepin (p<0.05).5. Wound Healing assay results showed that compared with the control group, gallbladder cancer cells showed significantly short transport distance when treated with 10μM and 20μM cordycepin, and showed a time-and dose-dependent (p<0.05); Migration assay results also showed that cell migration obviously reduced following treated with cordycepin.Conclusions:1. Cordycepin caused cell apoptosis and autophagy, and inhibit the proliferation of gallbladder cancer cells.2. Cordycepin significantly suppressed the cell migration of gallbladder cancer cells. |
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