The Microarray Comparative Genomic Hybridization Study Of Hepatocellular Carcinoma From Guangxi Zhuang Autonomous Region

Objective To know the characteristics of chromosome structure change in hepatocellular carcinoma (HCC) of Guangxi, combine with the bioinformatics, discoverying the oncogene and tumor suppressor gene in primary hepatocellular.Methods The cytogenetic change of 31 HCC was analysed by Microarray comparative genomic hybridization(Array CGH), combine with the bioinformatics, screening the genes with amplification and deletion (≥30%), to identify the genes that play a key role in the signaling pathway.Results DNA copy number changes of non-random in different degrees was found in the HCC.The regions with high frequency amplification(≥40%) include 1 q21.1-q32.1,8q22.2-q24.23; The regions with high frequency deletion (≥40%) include 1p34.2-p36.2,4q13.1-q35.2,8p11.22-p23.2,16q12.1-q24.3, 17p12-17p13.3,19p13.12-p 13.3; the DNA copy number changes higher than, 30% include the genes of TP53, CDH, MAP2K2, RB1, PIK3R5, EGF, NFKB1, TRADD, TNFRSF etc. And combine with the bioinformatics,the genes attend in 19 signaling pathways include apoptosis,,fas, ras-mediated of cell proliferation and DNA repair signaling pathway.Conclusion Screening the DNA copy number changes of 31 cases HCC by Array CGH, clarify the function of these genes in HCC occurrence and development, it apply to precancerous prevention, early diagnosis and a theory for screening the oncogene and tumor suppressor gene of hepatocellular carcinoma from Guangxi.