Effect Of Nicotinamide On Biological Behavior Of Lung Adenocarcinoma A549 Cells And Suggestions Of Possible Mechanisms

OBJECTIVETo investigate the effect of nicotinamide on the biological behavior of lung adenocarcinoma A549 cells and its possible mechanismMETHODSThe cultured A549 cells were treated with nicotinamide(10,20,30,40,50,60 mMol/L)for 24 h and 48 h, the control group was treated with cell culture media only. The morphologic changes of A549 cells were observed by inverted microscope.Cell counting of kit-8(CCK-8) assay was used to test the growth inhibitory rate and Annexin V-FITC/PI staining assay was applied to detect the apoptosis rate. The cell migration rate(12h,24 h,48h) were measured by the wound healing assay.All of the data was analyzed byone-way ANOVA and paired t-test with 95%confidence interval(CI) between the different groups.We also used Aligent human genome 4×44 microarrays to detect mRNA between A549 cells and A549 cells co-cultured with nicotinamide and screen significantly differential expressed genes in the role of nicotinamide,whose pathway were analyzed by KEGG Database.RESUITS The cell growth inhibition rate and cell apoptosis rate of A549 cells in experimental groups were higher than the control group in 24 h and 48h(P<0.05). Besides, the higher nicotinamide concentration and longer time of exposure had the higer cell growth inhibition rate and cell apotosis rate(P<0.05).The migration rate of A549 cells in experimental groups is higher than control group(P<0.05).In the group of A549 cells co-cultured withnicotinamide,there were 2505 differential expressed genes which were upregulated more than two fold change of the value and 745 differentialexpressed genes which were down-regulated more than two fold change of the value at the same time.And we screened the genes which was involved in cell proliferation and apoptosis pathway, such asBCL2.CONCLUSION Nicotinamide could significantly inhibit proliferation and promote apoptosis of A549 cells in a dose and time dependent manner, and it could also weaken the migration capacity of A549.The genes which was involved in cell proliferation and apoptosis pathway may be play a important rolein this process as the target,likeBCL2.However,thespecific mechanism of this process still need to be further studied.