Study On The Correlation Between Macrophage Migration Inhibitory Factor And Risk Factors Of Atherosclerosis In Pilots

Objective: To investigate the relationship between the plasma level of MIF and its related gene-173G/C polymorphism and risk factors of Atherosclerosis in pilots. In order to reduce the risk of adverse cardiovascular events in early stage.Methods: The collection of 750 subjects, divided into pilot atherosclerosis group(n=47), pilot health group(n=458), general atherosclerosis group(n=51) and general healthy group(n=194). General clinical data of the four groups were determined:age(Age), total cholesterol(TC), triglyceride(TG).The plasma level of MIF was measured with ELISA. The MIF- 173 G/C(rs755622) allele frequencies was detected by Taqman probe method, and respectively analyze the different genotype and allele frequency between the pilot atherosclerosis group and pilot health group, pilot atherosclerosis group and general atherosclerosis group, general atherosclerosis group and general healthy group. Statistical analysis using SPSS. Results: 1. The respectively plasma level of MIF in pilot atherosclerosis group, pilot health group,general atherosclerosis group and general healthy group were(323.47 + 6.101) pg /ml,(290.22 + 66.06) pg / ml,(291.08 + 82.87) pg / ml and(250.20 + 67.21) pg / ml.The plasma level of MIF in the pilot atherosclerosis group was significantly higher than that in the pilot health group(P < 0.05). The plasma level of MIF in the pilot atherosclerosis group was significantly higher than that in the general atherosclerosis group(P < 0.05). The plasma level of MIF in the general atherosclerosis group was significantly higher than that in the general health group(P < 0.05). 2. The CC, CG,GG genotype and C,G allele frequency in pilot atherosclerosis group were 2.1%,25.5%, 72.3%, 14.9%, 85.1%, in pilot health group were 2.8%, 31.9%, 65.3%, 18.8%,81.2%, in general atherosclerosis group were 5.9%, 19.6%, 74.5%, 15.7%, 84.3%. ingeneral atherosclerosis group were 2.1%, 33.5%, 64.4%, 18.8%, 81.2%. There was no significant difference in genotype and allele frequencies between the pilot atherosclerosis group and pilot health group(P>0.05). There was no significant difference in genotype and allele frequencies between the pilot atherosclerosis group and general atherosclerosis group(P>0.05). There was no significant difference in genotype and allele frequencies between general atherosclerosis group and general healthy group(P>0.05). Conclusion:1.There was no significant correlation between MIF-173G/C polymorphism and the early stage of Atherosclerosis. 2 Flight factors may lead to increased plasma MIF levels in pilots.3.Elevated plasma MIF levels may be associated with the development of Atherosclerosis.Objective: To investigate the effect of positive acceleration(+Gz)exposure on the expression of MIF in swine with different degree of coronary stenosis.To study the role of inflammation in cardiovascular injury induced by the +Gz stress.So as to provide theoretical basis for the flight safety of pilots with different coronary artery stenosis in flight. Methods: With 25 Bama miniature swines, the proximal left anterior descending branch(LAD) was ligated permanently by using silk suture under direct vision of the thoracoscope to establish different coronary artery stenosis models.Used coronary angiography results to determine the degree of stenosis. So as to dividid into sham operation group(n = 5, coronary artery without ligation), mild stenosis group(n = 7, coronary stenosis 20%-49%), moderate stenosis group(n = 6,coronary stenosis 50%- 69%), severe stenosis group(n = 7, coronary stenosis 70%-90%).+Gz exposure was performed in each group. Exposure to the initial +3Gz/60 s.G growth rate was 1G / s. each spin time interval was 10 min. the maximum +Gz was no more than +9GZ. using ECG monitored the risk electrocardiosignal(with the same lead continuous emergence of more than 3 times of the ventricular contractions before sexual period or ventricular fibrillation) as the standards to stop +Gz exposure, and the maximum +Gz tolerance was observed.Blood was collected at the time of 1minute before and after the exposure of the maximum +Gz acceleration tolerance in each group. Then, plasma were separated. The contents of MIF were measured by ELISA method. The expression levels of MIF were detected by RT-PCR and immunohistochemistry. Results: Compared with the sham operation group, the maximum tolerated +Gz values of moderate and severe stenosis group were significantly decreased(P<0.05), while the change was not obvious in mild stenosis group(P>0.05). After +Gz exposure, the expression of plasma MIF and MIF m RNA of Ascending aorta in the moderate and severe stenosis group were significantly higher than those in sham operated group(P<0.05), while the mild stenosis group was not significantly elevated(P>0.05). The expression of MIF protein was mainly localized in the cytoplasm, and the positive expression was brown yellow particles.Staining intensity: sham operation group and mild stenosis group(+), moderate stenosis group(+ +), severe stenosis group(+ + +). Conclusion: +Gz stress induced cardiovascular system damage of stenosis model of swine through activation of inflammatory response, and the degree of stenosis was more severe, the more intense the inflammatory response, the more espression of MIF, the maximum tolerated +Gz value was also more lower. +Gz exposure had effect on the expression of MIF in the different degree of coronary artery stenosis models, and the expression of MIF increased with the degree of stenosis.