The Function Of Cholic Acid In Liver Regeneration Of Obstructive Jaundice Mice Following Partial Hepatectomy

Objective:To evaluate the function of cholic acid in liver regeneration of obstructive jaundice mice following partial hepatectomy(PH) and its underlying mechanisms. Methods:160 male mice were randomly allocated into 6 groups:control group,normal mice subjected to PH(70% hepatectomy); obstructive jaundice(OJ) group, OJ mice without drainage subjected to PH;external drainage(ED) group,OJ mice with ED subjected to PH; ED+0.2% cholic acid(CA) group,OJ mice fed with 0.2%CA subjected to PH; ED+1%CA group, OJ mice fed with 1%CA subjected to PH; internal drainage(ID) group,OJ mice with ID subjected to PH. The liver regeneration rate and expression of Ki67, forkhead box M1b( Foxm1b) mRNA, fibroblast growth factor receptor 4(FGFR4) protein, and cell death rate with TUNEL of liver tissue were examined at 0,4,12,24,48,72 h after operation. Results: Ranking lists of the liver regeneration rate、positive rate of Ki67、relative quantitative expression of Foxm1 b mRNA and expression of FGFR4 of all groups: ID group>ED+0.2%CA group>ED group>OJ group>ED+1%CA group(P<0.05); There was no statistically significant difference between ID and Control groups in each index(P>0.05). Ranking list of the positive rate of TUNEL: ED+1%CA group>ED group>ED+0.2%CA group>Control group(P<0.05). Conclusions: ID is beneficial to liver regeneration after liver resection through reducing endogenous CA loss; Low concentration of exogenous CA supplement can improve the liver regeneration retardation caused by ED, which may be associated with its up-regulating Foxm1 b and FGFR4 expressions, and thereby promote liver regeneration.