Effect Of Recombinant Human Interleukin 11(rhIl-11) On Proliferation, Migration And Invasion Of Pulmonary Adenocarcinoma A549 Cells

Objective:To observe the effect of recombinant human interleukin 11(rhIL-11) on proliferation, migration and invasion of A549 cells and initially to investigate the mechanism of effect.Methods : We used the MTT assay to investigate whether the rhIL-11 effct on the proliferation of A549 cells and the wound-healing assay and transwell migration assay to validate the capability of the migration and invasion of A549 cells and Western Blot assays to investigate the effect of rhIL-11 on the expression of protein of MMP-2 and MMP-9 which correlative to invasion in A549 cells.Results:(1)The proliferation of A549 cells was not affected by rhIL-11.(2)rhIL-11 can promote the capability to migrate and invade of A549 cells with control groups in a dose-dependent manner( P<0.05).rhIL-11 increase the expression of the invasion MMP-9,MMP-2with a dose-dependent manner(P<0.05).Conclusions:(1)rhIL-11 has no definite effect on the proliferation of A549 cells.(2)rhIL-11 could promote the migration and invasion of A549 cells and up-regulation of MMP-2 andMMP-9 expression might be one of the mechanisms.