Combined Treatment Of ElectrospinningPHBV/PLA+COL Scaffold With Astrocyte In Rat After SCI

Objective:Combining Electrospinning PHBV/PLA+COL scaffold with AC, to study their effects, thereby determining the functionthey played on neuronal regeneration and recovery of SCI in rat.Methods:The growth and adhesion of AC in Electrospinning and PHBV/PLA+COL material are observed with electric scanning microscope and immunofluorescence, especially in the changes of gene expressions of BLBP, GLT-1, CSPG, S100-βmRNA after 7and 14 days’ in the materials.32 SD rats are randomly divided into 4 groups, namely, G1:controlledgroup, only transected; G2:AC transported; G3: PHBV/PLA+COL transported; G4:combining PHBV/PLA+COL and AC transported. The Basso, Beattie, and Bresnashan (BBB) scale system are used weekly to evaluate their motor recovery after surgery, and hematoxylin-eosin staining and neuroflament-200 (NF-200) and glial fibrillary acidic protein (GFAP) immunohistochemistry to evaluate the degree of injury of nervous tissue.Result:The reports from electric scanning microscope shows a great tendency of growth and adhesion of AC, and q-PCR indicates an significant increase in the number of BLBP, GLT-1 and S100-β. The BBB scale system tells that among the four groups, G4 has the highest score while G3 has a higher score than that of G1 and G2. At the same time, HE staining reveals the cavity is smaller that that of others, while the cavity in G1 being the biggest, far passing others. In addition,the GFAP and NF immunohistochemistry point out that there are glial scars in the boundary of the lesion, but the glial scar is smallest in G4, while those in G1 and G2 being the largest. Positive NF-200 nerve fiber is also growing more near the lesion, G3 coming as the second while little being seen in G1 and G2.Conclusion:The Electrospinning and PHBV/PLA+COL is biocompatible, which makes it a best candidate for scaffold. The Electrospinning and PHBV/PLA+COL scaffold loaded with AC can measurably reduce the inflammatory response in hemisection SCI rat, the formation of cavity and secondary damage to spinal cord, preventing the glial scar and promoting neuronal regeneration, thereby making the recovery of hind legs’ motor behavior possible.