Nuclear Factor I-C Inhibits Platelet-derived Growth Factor-induced Enhancement Of Dermal Fibroblast Sensitivity To TGF-β

Objective To investigate the effect of nuclear factor I-C (NFI-C) on platelet-derived growth factor (PDGF)-induced up-regulation of TGF-β receptor Ⅱ (TβRⅡ) in dermal fibroblasts.MethodsA lentiviral vector containing NFI-C sequence (Lenti-GFP-NFI-C) was transfected into a human foreskin fibroblast cell line (HFF-1). Cultured HFF-1 cells, cells transfected with Lenti-GFP-NFI-C, and cells transfected with a negative virus were stimulated with PDGF-BB, and Western blotting and RTqPCR were used to detect the expression levels of TPRⅡ in the treated cells.ResultsPDGF treatment significantly increased the expression level of TβRⅡ in HFF-1 cells (P<0.05). The cells transfected with Lenti-GFP-NFI-C expressed a significantly lower level of TβRII than non-transfected cells in response to PDGF stimulation (P<0.05), but the negative virus showed no such inhibitory effect (P>0.05). No significant difference was found in the expression level of TβRII protein between cells transfected with Lenti-GFP-NFI-C-transfection before PDGF stimulation and the blank control cells.ConclusionNFI-C can inhibit PDG-induced up-regulation of TβRII and thus reduce the sensitivity of the dermal fibroblasts to TGF-β.