Study On Chemical Components Of Ginkgo Biloba And Potential Mechanism For The Treatment Of Gout

Ginkgo(Ginkgo biloba L.) is one of the oldest tree species, the main active ingredients are flavonoids, terpenoids, organic compounds. They have the oxidation resistance, treatment of cardiovascular diseases, and so on. In this study, basing on the systems pharmacology to establish a chemical-target interaction model to investigate the mechanism of action for Ginkgo in treatment of gout by active component screening, target prediction, and chemical-target-gout network analysis, from molecular level, system comprehensive,integrated, in this paper, the active ingredient from ginkgo biloba leaves potential mechanisms for the treatment of gout. The main research contents and results include the following sections:1.This study uses normal phase silica gel, reversed-phase silica gel, Sephadex LH-20 column chromatography, thin layer of preparation of multiple chromatographic separation technology, with petroleum ether: ethyl acetate(2:8;1:9) as the elution system for the extraction of ethyl acetate by silica gel column chromatography, to obtain a higher purity of compound 1. Petroleum ether: acetone(7:3) as the elution system for the ninth part of the silica gel column chromatography, to obtain a higher purity of compound 3. Methanol: water(35%, 50%, 75%, 100%) as the elution system for the separation of the aqueous extract of Ginkgo biloba extract by SG64 macroporous adsorption resin. Chloroform: methanol(9:1,13:2, 5:1, 8:2) for the preliminary separation eighteenth part of the silica gel column chromatography, to obtain a higher purity of compound 2. We use nuclear magnetic resonance13C-NMR and 1H-NMR detection technology, and reference in the literature of the nuclear magnetic information. The results show that compound 1 is kaempferol, compound 3 is ginkgolide B, Compound 2 is a new compound2-{4-[4-(3,5-Dihydroxy-phenyl)-tetrahydro-furo[3,4-c]furan-1-yl]-2,6-dimethoxy-phenoxy}-6-hydroxymethyl-tetrahydro-pyran-3,4-diol, molecules are C26H31O11.2.This study uses systems pharmacology approach, oral biological utilization degree(OB) measure the chemical constituents from the leaves of Ginkgo biloba in the vivo pharmacokinetics and drug, drug likeness(DL) to predict the similarity of Ginkgo biloba chemical components with known drug, through OB≥30 and DL≥0.18 as a filter condition,A total of 24 potential active compounds include 11 flavonoids and 7 terpenoids were identified from 158 known compounds of ginkgo biloba, along with 49 potential targets.Through the network(compound target) and pathway analysis to explain active compounds of ginkgo biloba may be through three ways to cure gout: First, minimize the production of uric acid. Second, increase the excretion of uric acid. Third, inhibit NALP3 inflammation caused by disorders.The method and the result of this study are provided theoretical basis for further experimental studies and the direction, are provied for the development of more effective gout drug resistance to provide the scientific basis.