Study The Effect Of Solanesol Anti-inflammatory And Antioxidant On Experimental Periodontitis In Rats

Objective:The aim of the study is to investigate the effect of the solanesol anti-inflammatory and antioxidant on experimental periodontitis in rats by using internal and external inflammation model.Methods:1. In vivo experiment:One hundred and twenty-six healthy male SD rats were randomly divided into normal group(N,18 rats), periodontitis model group(108 rats). Using ligating floss around second maxillary molars of cervical with high-sugar soft diet for 4 weeks induce experimental periodontitis model. After the models were completed, the periodontitis rats were randomly divided into six groups(18 rats/group), contained Periodontitis model group (P), Tinidazole group (T) and Vitamin C group (VitC), Solanesol low dose group (S1), Solanesol medium dose group (S2), Solanesol high dose group (S3). The rats were intragastrical administrated with different drugs. Tinidazole group by intragastric administration with 200 mg/kg on the first day, the next 100 mg/kg of Tinidazole solution; Vitamin C group by intragastric administration 100 mg/kg Vitamin C solution; Solanesol in low, middle and high group by intragastric adiministration with 1.5,3.0,6.0 mg/100g of solanesol solution respectively. N and P group by intragastric adiministration with the same amount of oil. Each group were randomly selected 6 rats at three different time points (2w,4w and 6w), blood samples were collected from abdominal aorta, then the rats were executed. The maxilla were isolated and the left side was used to measure the loss of alveolar bone. The right side of the rat was stained with HE and TRAP. ELISA was used to detect the plasm expression level of inflammatory cytokines as IL-1β, TNF-a and PGE2. Hydroxylamine and DTNB were used to detect the plasm activity of SOD and GSH-Px. TBA was used to detect the content of MDA.2. LPS(1mg/L) was used to stimulated macrophage RAW264.7 to establish inflammatory model in vitro. MTS assay was used to detect the cytotoxicity of solanesol on RAW264.7 cells. Then the cell inflammatory model was used to investigate the effect of the solanesol anti-inflammatory and antioxidant. The experimental group was divided into Negative control group; LPS group; LPS+VitC group; LPS+VitE group; LPS+solanesol group with different concentrations(3.75uM、 7.5uM、15uM、30uM、60uM). The cell culture supernatant was collected, ELISA method for the detection of inflammatory factor IL-1β、 TNF-a and PGE2 content. The cell was collected, Hydroxylamine and DTNB were used to detect the activity of SOD and GSH-Px. TBA was used to detect the content of MDA. The above experimental data were analyzed by using the SPSS17.0 data statistical software.Results:1.After modeling for 4 weeks, a large number of inflammatory cell infiltration in periodontal tissues, epithelial spikes elongate, irregular resorption of alveolar bone and periodontal pocket formation, which indicated periodontitis model establishment. After drug intervention for 2W,4W,6W, the alveolar bone loss of each group was reduced, and the extent of osteoclast activity decreased. However, no significant difference between treatment groups and periodontitis group(P>0.05). Change of inflammatory factors and indexes about oxidative stress in plasma: compared with the normal group, periodontitis group IL-1β, TNF-a, PGE2 expression were significantly increased (P<0.05). Compared with the periodontitis group, the treatment group were significantly reduced IL-1β, TNF-a, PGE2 expression (P<0.05). Meanwhile, SOD, GSH-Px activity was increased, and MDA content was reduced (P <0.05).2. After lmg/L LPS stimulated RAW264.7 macrophages with 24h, the expression of inflammatory factor IL-1β、TNF-a and PGE2 and the content of MDA were significantly increased, the activity of SOD and GSH-Px were decreased. Different concentrations of Solanesol decrease expression levels of IL-1β, TNF-a, PGE2 in a dose-dependent manner. Solanesol also increased the antioxidant enzymes SOD and GSH-Px activity and reduced MDA content.Conclusion:Treatment with solanesol can significantly reduced expression levels of IL-1β, TNF-a, PGE2 and the content of MDA, increase the activity of SOD and GSH-PX. But the effect of solanesol reducing the alveolar bone loss and inhibiting osteoclast activation is not obvious.