Serum Levels Of Interleukin-33 And Osteopontin In Patients With Systemic Lupus Erythematosus And Sj(?)gren’s Syndrome

Objective:To determine the levels of interleukin (IL)-33 and osteopontin (OPN) in the serum of Chinese patients suffering from systemic lupus erythematosus (SLE) and Sjogren’s syndrome (SS)Methods:This case control study was conducted with 60 Chinese individuals of both genders, over the age 16 to 74 with the median of 41 years, who were divided into four groups; (1) the SLE group (n= 31), (2) the Sjogren’s syndrome group (n= 8), (3) the SLE/Hashimoto’s thyroiditis group (n= 12), and (4) the healthy control group (n= 9). A Quantikine ELISA human IL-33 immunoassay and Quantikine ELISA human osteopontin immunoassay reagents were used for the analysis of the serum concentration. All the patients were recruited from the Rheumatology and Clinical Immunology Clinic at the Second Affiliated Hospital of Kunming Medical University.Results:We observed that there is not significant relationship between serum IL-33 and OPN with the age among the 4 groups. In the analysis of serum IL-33 levels in between the 4 groups, the observed values of F and P were 0.144 and 0.933 respectively. Here the observed value of P is greater than 0.05 (P>0.05). Hence, there was no significant difference between the 4 groups in IL-33 levels. In the analysis of serum OPN levels between the 4 groups, the observed values of F and P are 0.4602 and 0.006 respectively. Here the observed value of P is less than 0.05 (P<0.05). Hence, there is significant difference between the 4 groups in OPN levels. As there was a significant difference between the groups on OPN level, a multiple comparisons was done between the 4 groups. From the comparison we observed serum concentration of OPN level was significantly higher in SLE with Hashimoto’s thyroiditis than other 3 groups.In SLE, the analysis we observed that p-value is greater than 0.05 (P>0.05) except positive Anti-SSA (P=.0390), there was no significant relationship between disease activity except positive Anti-SSA antibody. Most clinical and laboratory variables did not correlated with serum IL-33 and OPN levels. There was a higher serum IL-33 level in patients with SLE having Anti-SSA positivity but there is not any relation between OPN level and clinical manifestation.In Sjogren’s syndrome, the analysis we observed that p-value is greater than 0.05 (P>0.05) except positive Anti-SSB antibody (P=0.036), there was no significant relationship between disease activity except positive Anti-SSB antibody. Most clinical and laboratory variables did not correlate with serum IL-33 and OPN levels. There was a higher OPN levels in patients with Sjogren’s syndrome with Anti-SSB antibody positivity but there was no any relation between IL-33 levels and clinical manifestation.Conclusion:This study concluded that there was no significance difference between the 4 groups in IL-33 levels. There is a significant difference between the groups on OPN levels from the multiple comparisons between the 4 groups we observed that serum concentration of OPN level is significantly higher in SLE with Hashimoto’s thyroiditis than other 3 groups.