| [Objective] The osteogenic performance of porous HAPw/n-ZnO composite material in the case of in-animal bone defect and its repair performance in the case of infectious bone defect had been studied through animal experiment.[Methods] 48 normal SD rats(male) were selected and randomly divided into two groups with 24 rats for each group, an annular bone defect which was 3mm both in diameter and depth had been prepared at the lateral femoral condyle of the rats’left and right posterior limbs, the rats in group one had been implanted with porous HAPw/n-ZnO composite material at the bone defect of left posterior limb(group A) while without being implanted with it at the counterpart of right posterior limb (blank group); the rats in group two had been implanted with porous HAPw/n-ZnO composite material containing BMP at the bone defect of left posterior limb(group B) and Bio-Oss bone powder at the counterpart of right posterior limb(group C).8 rats in each group had been killed respectively at week 6,12 and 18 after the operation, a general observation, Micro-CT test and histopathological observation had been performed to investigate the osteogenic performance of porous HAPw/n-ZnO composite material in the case of in-animal bone defect.42 normal SD rats were selected, in view of the accidental factors including the anesthetic accident and the infection and death due to individual difference and so on,39 rats were actually effective, an annular bone defect had been prepared at the lateral femoral condyle of the rats’ left and right posterior limbs and a staphylococcus aureus solution (1×104CFU/ml, ATCC6538) had been placed into the bone defect, they were randomly divided into three groups 3 weeks later:group A (n=15):porous HAPw/n-ZnO composite material; group B(n=15):Bio-Oss bone powder and a blank control group(n=9). The rats in all groups were killed respectively at week 2,6 and 8 after the operation for general observation, Micro-CT test and histopathological observation, which were used to investigate the performance of anti-infection bone repair of porous HAPw/n-ZnO composite material in the case of infectious bone defect of animal.[Results] Experiment No.1, it was shown by HE staining that, group A, B and C were better than the blank control group in terms of bone repair capability, the group C has the best osteogenic performance, group A was not significantly different from group B, it was shown by the Micro-CT test that, group C presented the best testing parameters in each item, and it has a statistical significance as compared to other three groups (P<0.05), while the difference between group A and B was not statistically significant (P>0.05).experiment No.2:it was shown by HE staining that, the repair effect on inflammation of group A was clearly better than that of group B and blank control group in 2 weeks, a small amount of newly born capillaries and fibrous connective tissues had been formed, the inflammatory cellular infiltration was not obvious, a new bone formation was presented in 8 weeks; it was shown by a bacteriological test that, there was a diffused distribution of a few bacterial clumps on the culture dish of group A, a distribution of a lot of bacterial clumps can be seen on the culture dish of group B and blank control group, and group A equally had a statistical significance as compared to the other two groups, while the group B was not found to be significantly different from blank control group.[Conclusion] Porous HAPw/n-ZnO composite material has a certain effect of osteogenesis and anti-infection on the bone defect in animal’s body, among which, Bio-Oss bone powder does a better job than Porous HAPw/n-ZnO composite material in osteogenesis, while the Porous HAPw/n-ZnO composite material precedes other materials in anti-infection. |
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