| Objective: To observe the effects of Tanshinone IIA sodium sulfonate(TSS) on ischemia/reperfusion(I/R) induced cardiac injury in male(Sprague-Dawley, SD) and explore its mechanisms.Method: Rats were subjected to a 30-min coronary arterial occlusion followed by 24 hours reperfusion. The survival rats were randomly divided into sham group(sham group, n = 10), ischemia reperfusion group(I/R group, n = 10), low dose of sodium tanshinone IIA sulfonate group(TSS-L group, n = 10), medium dose of tanshinone IIA sulfonate sodium group(TSS-M group, n = 9), high dose of sodium tanshinone IIA sulfonate group(TSS-H group, n = 9). A MAP heart function analysis system was used to detect measure hemodynamic parameters, TTC staining method was used to detect the myocardial infarct size. The expression of Bcl-2, Bax, Caspase-3, Lc3B/Lc3 A, Beclin-1 and high mobility group box1(HMGB1) were detected by western blot method. All data were analyzed by using One-way analysis of variance(ANOVA) or student t test.Results: Cardiac function in I/R group was lower than that in sham group, which were significantly improved by pretreated with TSS(F=98.329, P<0.05), but Pmin and DAP had no significant difference(P>0.05). The percentage of myocardial infarct size in TSS pretreatment group was significantly lower than that in I/R group(F=428.684,P<0.05). Compared with sham group, not only were the levels of Caspase-3 and Bax increased, But also the Bcl-2 content was reduced obviously(t=-5.091, t=-6.521, t=0.946,P<0.05, respectively). TSS group significantly down regulate Caspase-3 and Bax protein(F=16.113, F=12.823, P<0.01, respectively). At the same time, all dose groups of TSS pretreatment increased the level of Bcl-2(F=22.921, P<0.01). Compared with sham group, the ratio of Bcl-2/Bax in I/R group was lower(F=37.253,P<0.05);which was elevated in TSS groups(F=18.297,P<0.05). The trend of the change of Autophagy related protein beclin-1 and Lc3B/Lc3 A was similar, the level of beclin-1 and Lc3B/Lc3 A in I/R group were lower that in sham group(t=4.886,t=5.785,P<0.05, respectively), which were raised in TSS pretreatment groups(F=48.809,F=11.475,P<0.05). The expression of HMGB1 in I/R group was higher than that in sham group(t=8.591, P<0.05), and compared with I/R group, pretreated with TSS decreased the expression of HMGB1 significantly(F=25.338,P<0.01).Conclusion:(1).TSS could ameliorate I/R induced myocardial injury and improve cardiac function, and the effect was in a dose-dependent manner in a certain range.(2) The protective effect of sodium IIA sulfonate sodium on myocardial ischemia reperfusion injury in SD rats may via reducing inflammation and apoptosis and enhancing autophagy. |
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