The Correlation Between RASSF1A 133 Single Nucleotide Polymorphism And Non-small Cell Lung Cancer

Objective: The Ras-Association domain Family1(RASSF1)is a tumor suppressor gene(TSG). The aim of current study is to investigate the relationship between a single nucleotide polymorphism(SNP) at codon 133 of the RASSF1 A gene and non-small cell lung cancer(NSCLC) susceptibility,clinical parameters and survival.Material and methods:225 pathologically diagnosed NSCLC patients and 225 healthy controls from Shanghai Pulmonary Hospital were enrolled. Clinicopathological features were collected and follow-up. The method of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to explore the polymorphism at codon 133 in the RASSF1 genes. EGFR mutation status of NSCLC patients was detected by ADx Amplification Refractory Mutation System(ADx-ARMS).Results: Compared to healthy controls, the risk of Ala/Ser and Ala/Ser + Ser/Ser genotype were increased in NSCLC patients(Adjusted P =0.028, Adjusted P =0.013). The Ala/Ser genotype was associated with male(adjusted OR=3.84, 95% CI: 1.407-10.482) and smoking status(adjusted OR=11.967, 95% CI: 2.195-17.475), otherwise no significant difference with EGFR mutation. The RASSF1 A SNP indicated a poor prognosis.Conclusions: RASSF1 A Ala133Ser SNP contributes to non-small cell lung cancer susceptibility and poor prognosis. The relevance between RASSF1 A Ala133Ser SNP and male, smoking status is also close. No significant difference between RASSF1 SNP and EGFR mutation.