| Purpose:18F-FDS (18F-2-fluorodeoxy sorbitol) is a new PET (positron emission tomography) tracer and has the potential to specifically detect Enterobacteriaceae infections. The purpose of this study was to discuss the efficacy and safety of 18F-FDS.Methods:FDG-N and multifunctional synthesis module were employed for the fully automated radiosynthesis of 18F-FDS. Quality control was performed before clinical use. Biodistribution in vivo and 18F-FDS MicroPET imaging were carried out on normal babl/c mice (n= 25), E. coli infected mice (n= 3), and sterile mice (n= 3). Antibiotic treatment was also evaluated.18F-FDS and 18F-FDG imaging on tumor-bearing mice (n= 9) were compared. Dynamic 18F-FDS (320～520 MBq) PET/CT scans were performed on six healthy subjects. Radiation dosimetry was calculated via OLINDA/EXM.Results:The overall radiochemical yield of 18F-FDS was 40 ± 4%(n= 8) with a total synthesis time of 45 min, radiochemical purity> 99%, PH 6.5～7.5, the probe remained greater than 95% intact in fetal bovine serum after 120 min. MicroPET imaging suggested that 18F-FDS was excreted mainly through the urinary system. Background uptake was relatively low. The E. coil infection lesion showed high uptake, T/NT ratio was 12.4 ± 0.44 at 60 min. After antibiotic treatment 24 h, a significant decreased was detected (3.05 ± 0.19, P< 0.05). Compared to 18F-FDG, a much lower T/NT ratio was observed on tumor-bearing mice (P< 0.05). In human studies, rapid clearance through urinary system was. About 35% of the injected activity (decay-corrected) was eliminated through the kidneys within the first hour. The effective dose (ED) was estimated to be 0.021 ± 0.001 mSv/MBq.Conclusion:18F-FDS was easy to synthesize with high yield. It could be used to specifically detect E. Coli infection in animal experiment. The ED of 18F-FDS was well within the regulatory limits for human research. |
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