Effect Of HSP22 Over Expression In Pd Trans Genic Drosophila Model Mediated Mitochondr Ial Unfolded Protein Response

Objective: To study the effects and mechanism of HSP22 over expression in PD transgenic drosophila model,provide the basis for the further reserch of pathogenesis of PD. Methods: The PD transgenic drosophila model is intervened by HSP22 OE, GCN2 RNAi and the equilibrium systerm of HSP22 OE; GCN2 RNAi. The effects of phenotype on drosophila were observed by the flight test. The expression of phosphorylation of AMPK and eIF2αwas detected by Western Blot. Results: The rate of wing-phenotype of PD transgenic drosophila genetic intervention of GCN2 RNAi was significantly reduced(P<0.005), and the jump/flight ability was slightly increased(P<0.05); the expressions of P-AMPK and P-eIF2αwere both downregulated.The rate of wing-phenotype of PD transgenic drosophila genetic intervention of HSP22 OE was significantly reduced(P<0.001), with the jump/flight ability was also significantly increased(P<0.001); the expression of P-AMPK and P-eIF2αwere both downregulated significantly. The rate of wing-phenotype of PD transgenic drosophila genetic intervention of the equilibrium systerm of HSP22 OE; GCN2 RNAi was significantly reduced(P<0.005), and the jump/flight ability was slightly increased(P<0.05);but the expression of P-AMPK and P-eIF2αwere both upregulated. Conclusions:(1) The excessive reaction of UPRmt may be one of the pathogenic factors in PD transgenic drosophila.(2) HSP22 has a protective effect on PD transgenic drosophila, and its protective mechanism may be negatively correlated with the regulation of UPRmt stress level.