The Study Of The Changes Of Peripheral Th17 Cells And Treg Cells And Th17/Treg Balance In Patients With Chronic Hepatitis B Virus Infection

Objective:In this research, we select the HBV-related liver diseases patients as experimental group, including asymptomatic carriers (ASC), chronic hepatitis B (CHB), liver cirrhosis (LC), hepatocellular carcinoma (HCC),and set the healthy adults (HC) as the control group, To investigate the changes of peripheral Th17 cells and Treg cells and Th17/Treg balance in patients with chronic hepatitis B virus infection, and to exploring the possible role in development process of the disease of chronic HBV infection, and to provide a theoretical basis to elaborate the mechanism of the chronicity about HBV infection and its progression.Methods:In this research, we selected 142 cases of HBV-related liver diseases patients, who came from inpatient or outpatient in infectious diseases department of our hospital from February 2014 to November 2015. Among them,23 cases were ASC,51 cases were CHB,37 cases were LC,31 cases were HCC, and set 26 cases whose physical examination were normal as the control group. The frequency of Thl7cells and Treg cells in the peripheral blood of the patients group and control group were detected by flow cytometry. The HBV-DNA were tested by real-time fluorescent quantitative PCR analyzer. The ALT, AST and TBil were detected by the automatic biochemical analyzer. All of these data were analyzed by the SPSS 17.0 statistical software.Results:1)About the Th17/CD4+T:With the analysis of variance, these five groups have statistically significant (F=27.934, P<0.05). By further pairwise comparison, we find the group of LC(4.42±1.69) is higher than the group of HC(1.59±0.65), ASC(1.34 ±1.35), CHB(3.46±1.37), HCC(2.56± 1.17), and the difference is statistically significant(p<0.05). The group of CHB(3.46±1.37) is higher than the group of HC(1.59±0.65), ASC(1.34±1.35), HCC(2.56 ±1.17), and the difference is statistically significant(p<0.05). The group among HC, ASC and HCC, there has no statistical significance(p>0.05).2) About the Treg/CD4+T:With the analysis of variance, these five groups have statistically significant (F=31.403, p<0.05). By further pairwise comparison, we find the group of HCC(3.39±0.72) is higher than the group of HC(1.15±0.53), ASC(1.29 ±0.79), CHB(2.36 ± 0.79), LC(2.55 ± 1.21), and the difference is statistically significant(p<0.05). The group of CHB(2.36±0.79) and LC(2.55±1.21) is higher than the group of HC(1.15±0.53) and ASC(1.29±0.79), and the difference is statistically significant(p<0.05). The group between HC and ASC, CHB and LC, there has no statistical significance(p>0.05).3) The ratio of Th17/Treg:With the analysis of variance, these five groups have statistically significant (F=6.074, P<0.05). By further pairwise comparison, we find the group of LC(2.11 ± 1.28) is higher than the group of HC(1.29±0.98), ASC(1.37 ±1.71), CHB(1.64±0.99), HCC(0.78 ± 0.37), and the difference is statistically significant(p<0.05). The group of HCC(0.78 ± 0.37) is lower than the group of HC(1.29±0.98), ASC(1.37±1.71), CHB(1.64±0.99), LC(2.11 ± 1.28), and the difference is statistically significant(p<0.05). The group of CHB(1.64 ±0.99) is higher than the group of HC(1.29±0.98) and ASC(1.37± 1.71), and the difference is statistically significant(p<0.05). The difference between the group of HC and ASC has no statistical significance(p>0.05)4) The correlation analysis of Th17, Treg, Th17/Treg and liver function:In group of CHB, Th17 is positively correlated with ALT(r=0.634xx, p<0.05), but has no correlation with AST and TB, Treg has no correlation with ALT, AST and TB, the ratio of Th17/Treg is positively correlated with ALT(r=0.630.xx, p<0.05), but has no correlation with ALT and AST. In group of LC, Th17 is positively correlated with ALT(r=0.332xx, p<0.05), but has no correlation with AST and TB, Treg is positively correlated with ALT(r=0.525xx, p<0.05), but has no correlation with AST and TB, the ratio of Th17/Treg has no correlation with ALT, AST and TB. The liver function has no correlation with Th17, Treg, Th17/Treg in group HC, ASC and HCC.Conclusion:1)Th17 and Treg in patients with chronic HBV-infected liver is in a relatively active state, to a certain extent, it is positively correlated with the liver function. 2)The process from CHB to LC is an inflammatory process of constant development..3)Th17 cells and Treg cells has closely relationship with the occurrence and progress of HCC.