Analysis Of Antibiotics-resistance Of Propionibacterium Acnes From Some Acne Patients In Yunnan Area And The Inhibitory Effect Of Polyphylla Saponins On Acne-related Pathogens

Objectivesr:1.To study the antibiotics-resistance of some acne patients in Yunnan area. 2.To research the inhibitory effect of polyphylla total saponins and polyphylla Ⅰ, Ⅱ, Ⅲ, Ⅴ, Ⅵ, Ⅶ, H on acne-related pathogens (Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus).Methods:1. Seventy cases of acne patients in Yunnan area was collected, isolating the lesional extrudates anaerobic cultured for 72h, then according Gram’s staining and VITEK2-compact automatic microbial analysis system appraised of Propioni-bacterium acne, according E-test s inhibition ring size to evaluated the antibiotic resistance situation (According to the CLSI 2011M100-S21 standard for analysis). 2.Firstly, liquid microdilution method (96 pore plate fold dilution method) was used to observe polyphylla total saponins on Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus’s minimal inhibitory concen-tration (MIC) to evaluate whether total saponins has bacteriostatic action,and thentesting the MIC of different polyphylla components on the three kinds of pathogenicbacteria,to evaluate which saponins has explicit antibacterial effect.Results:1.After identification, twenty-eight case of acne patients’lesional were isolated and cultured for Propionibacterium acnes, the positive rate was 40%.2.Tetracycline, doxycycline, cefaclor of P.acnes is sensitive (sensitive rate is 96%, 100% and 100% respectively). Among them,tetracycline, doxycycline is more sensitive, the MIC values were between the 0.016-32,0.016-3.Oug/ml respectively; clarithromycin, erythromycin, clindamycin, azithromycin sensitive to few acne patients(sensitive rate is 25%,25%,17.9%,17.9% respectively), the MIC values were between the 0.016～>256ug/ml; and metronidazole, mupirocin on all subjects acne patients were no bacteriostasis, means resistance.3.MIC of polyphylla total saponins on Propionibacterium acnes NCTC737, ATCC6919, Staphylococcus epidermidis ATCC12228, Staphylococcus aureus ATCC6538 is 2.5,5.0,5.0,1.25mg/ml respectively;polyphylla Ⅰ, Ⅱ,Ⅲ, Ⅴ, Ⅵ, Ⅶ, H on Propionibacterium acnes NCTC737 strain’s MIC were 0.625,1.25,2.5,5,2.5,2.5, 10.0mg/ml;on ATCC6919 strain’s were 1.25,1.25,5,5,2.5,2.5,10.0mg/ml;on Staphylococcus epidermidis ATCC 12228 strain’s MIC were 1.25,1.25,5,5,2.5,2.5, 10.0mg/ml;on Staphylococcus aureus ATCC6538 strain’s were 0.625,0.625,2.5,5, 2.5,2.5,10.0mg/ml;the positive control of erythromycin on Propionibacterium acnes NCTC737, ATCC6919 ATCC 12228 and Staphylococcus epidermidis, Staphylococcus aureus ATCC6538 MIC were 0.05,0.05,0.0125,0.025 mg/ml; the positive control of clindamycin on Propionibacterium acnes NCTC737, ATCC6919 and ATCC 12228 of Staphylococcus epidermidis and Staphylococcus aureus ATCC6538 MIC were 0.025, 0.025,0.00625,0.0125 mg/ml.Conclusions:1. In the part of acne patients in Yunnan Province of this research, the commonly used antibiotics in treating of acne, metronidazole, mupirocin highly resistant, most of erythromycin, azithromycin, clindamycin, clarithromycin resistance, tetracycline, doxycycline, cefaclor sensitive. Therefore, in the guidance of acne patients should avoid used the prone to resistance of P.acne’s antibiotic,but used more sensitive antibiotics,such as astetracycline, doxycycline, in order to improve the clinical efficacy.2.Polyphylla total saponins has a good inhibitory effect on acnes related pathogens; polyphylla Ⅰ, Ⅱ, Ⅵ, Ⅶ in vitro on Propionibacterium acnes related pathogens have an explicit inhibitory effect, which polyphylla I’s inhibitory effect is the strongest.