MiR149 And Androgen Receptor Have An Effect On Castration Resistance In Prostate Cancer

Objective: Study the relationship between mi R149, androgen receptor(AR) and castration resistance in prostate cancer.Method: Culture LNCa P and PC-3 cells which are androgen-dependent and androgen-independent prostate cancer cells. Observe the growth of LNCa P, PC-3 cells under the condition of androgen deprivation. Progressively decrease the androgens in LNCa P cells to gain LNCa P-A1 cells which are androgen-independent. Test the content of AR and mi R149 in LNCa P and PC-3, LNCa P and LNCa P-A1 cells by Western-blot and real-time PCR.Results: Under the condition of 50% initial androgens, LNCa P cells began to be suppressed. The inhibition was more obvious with the androgen decreasing. When the androgen was totally deprived, the LNCa P cells quickly underwent apoptosis. PC-3 cells can grow normally in every level of androgens. According to the results of Western-blot and real-time PCR, LNCa P cells had a higher expression of AR and a smaller content of mi R149 compared to PC-3 cells. And LNCa P cells had an increased AR level and decreased mi R149 content when transformed to LNCa P-A1 cells.Conclusion: The increased expression of AR is one of the important reasons for castration resistant. Reduced mi R149 content may be a reason to the amplification of AR, suggesting that mi R149 can suppress the m RNA of AR. Promoting the expression of mi R149 may restrain the process of castration resistance in prostate cancer.