Analysis Of Saliva Microbiota Between Chronic Periodontitis Patients And Periodontal Healthy People

Objective: Analyzing the saliva microbial community structure and difference between chronic periodontitis patients and periodontal healthy people. In order to explore chronic periodontitis pathogens and to provide experimental support for microbial pathogenesis further study.Methods: According to the WHO sampling standards, 47 saliva samples were collected samples which included 24 chronic periodontitis patients and 23 healthy people from linxia, Gansu province, and marked P(chronic periodontitis patients) and H(healthy people). Saliva samples’ DNA was extracted. The V4V5 region was amplified by PCR and tested, Followed it was sequenced by GS 454 FLX+. Unqualified sequences were eliminated. Finally, softwares and related databases such as Qiime、MOTHUR、RDP、NCBI and MEGA were used to analysis and get microbial taxonomy information.Results:(1) 2272 OTUs were detected from saliva samples of these two groups, attributed to the13 phyla, 22 classes, 39 orders, 80 families and 159 genera.(2) 13 phyla were detected in both P and H group: Firmicute, Proteobacteria, Actinobacteria,Bacteroidetes, Fusobacteria, Synergistetes, Chlamydiae, Chloroflexi, Spirochaetes, Tenericutes,GN02, SR1 and TM7. The dominant bacteria phyla included: Firmicute, Proteobacteria,Actinobacteria, Bacteroidetes, Fusobacteria and TM7. Wilcoxon test for the abundance of 13 phyla showed that no statistical significance(p> 0.05) between two groups.(3) There were 11 dominant genera in P and H group, included: Actinomyces(P: 3.94%, H:3.66%), Porphyromonas(P:4.33%, H: 4.13%), Prevotella(P: 6.96%, H: 3.60%), Gemella(P:4.70%, H: 5.56%), Granulicatella(P:4.54%, H: 7.90%), Lactobacillus(P:4.11%, H:1.67%),Streptococcus(P:36.35%, H: 36.84%), Fusobacterium(P:4.42%, H: 3.52%), Leptotrichia(P:1.47%, H: 1.32%), Neisseria(P:8.86%, H:12.68%), and Lautropia(P:1.59%, H: 2.36%).Except Veillonella, Capnocytophaga, Rothia and TM7_[G-1]. Granulicatella and Veillonella are dominant genera.(4) 31 genera had a significant difference between H and P group. The content of Atopobium,Cryptobacterium, Bacteroidetes_[G-3], Mitsuokella, Lachnospiraceae_[G-5], Shuttleworthia,Parvimonas,Peptostreptococcaceae_[XIII][G-1], Anaeroglobus, Agrobacterium,Ottowia, and TM7_[G-5] are relatively high in P group.(5) RDA analysis revealed that Clostridiales_[F-3][G-1], Lachnospiraceae_[G-6] may correlate with periodontal pathogens, and their OTU content <0.01%.Conclusion:(1) There are some differences of oral microbial community structure between chronic periodontitis patients and periodontal healthy people.(2) The OTU abundance of the phylum is different but the species may constant between chronic periodontitis patients and periodontal healthy people.(3) There may exist "Oral core microbiome" in chronic periodontitis and periodontal healthy population saliva microorganisms.(4)The genera of OTU content <0.01% may affect the oral microbial community structure,especially Clostridiales_[F-3][G-1] and Lachnospiraceae_[G-6].