The Experiment Research On Proliferation In Hepatic Carcinoma Cell Through JNK Signaling Pathway Via MIF

Objective To Elucidate the molecular mechanisms of the hepatocellular carcinoma proliferation through JNK signaling pathway, one of the MAPK signaling pathways, via MIF. And to provide a theoretical basis for exploring drug research and development of hepatocellular carcinoma with therapeutic target in MIF.Methods First, added respectively 0, 50, 100, 150, 200 ng/ml concentration of MIF in to Hep G2 and L-02 cells as experimental intervention and control groups, and added MIF inhibitor ISO-1(50ng / ml) interventing Hep G2 cells as a negative control group, and used MTT assay to detect the cells proliferation. Secondly, set Hep G2 and L02 cells respectively by ISO-1 and 0, 100, 200 ng/ml concentration of MIF to culture 48 hours. The cells in each group were cultured for 48 hours. Collected the cells, detected the nucleic acid expressions of JNK in Hep G2 cells by the method of q RT-PCR, and detected the protein and the phosphorylation protein expressions of JNK in Hep G2 cells by the method of western blot.Results We tested the effect of MIF on Hep G2 cell proliferation through MTT assay. And with the increase of MIF concentrations, the promoting effect to the proliferation of Hep G2 cells is more obvious; The experimental group compared with the negative control group and blank control group, it has significant statistical significance(P <0.05). We detected that MIF has significant roles of promoting the expression of the phosphorylation JNK by method of q RT-PCR and Western blot; the experimental group compared with the negative control group and blank control group, it has significant statistical significance(P <0.05).Conclusion 1. MIF can promote hepatocellular carcinoma cell proliferation, and has a concentration-dependent manner. 2. MIF may promote the proliferation of Hep G2 cells via JNK signal transduction pathway.