A Research Of The NF-kB Pathways Through Which Bone Marrow Mesenchymal Stem Cells-derived Microvesicles Act On Chondrocytes

Objective 1. Institute(bone marrow mesenchymal stem cells, BMSCs), study and improve bone marrow mesenchymal stem cells derived micro vesicles(bone marrow mesenchymal stem cells-derived microvesicles, BMSCs-MVs) separation, extraction, identification methods; 2, study of bone marrow mesenchymal stem cells derived micro vesicles(BMSCsMVs), a regulatory role in osteoarthritis(Osteoarthritis, OA) and to explore the molecular mechanisms governing NF-KB pathway signaling pathway.Methods: 1. in vitro culture environment and the timely transfer between generations SD rat bone marrow mesenchymal stem cells BMSCs; 2. From the bone marrow in vitro of SD rat mesenchymal stem cells BMSCs by ultracentrifugation extracted and amplified BMSCs-MVs, corresponding cytological, morphological identification; 3. Environment and cultured in vitro passage experiments chondrocytes SD rats, and the corresponding cytological, morphological identification, provide some source for the next step to continue the experiment; 4. BMSCs-MVs and SD rats were cultured chondrocytes after timely passage, extracted total RNA, RT-PCR was detected by reverse transcription-related factors after using SPSS 16.0 statistical analysis. Further inquiry BMSCs-MVs on chondrocytes proliferation and apoptosis in the direction of respect and BMSCs-MVs role in signaling pathways chondrocytes NF-kb transduction mechanisms;Results The test results using software SPSS16.0 processing and analysis, the results were expressed as mean ± standard deviation expressed as relative expression values of different experimental treatments target genes were used for independent samples t-test were analyzed, the results of post-processing software p <0.05, meaning that the difference was statistically significant experimental data. 1. Each experimental group and control group were compared, the expression of pro-apoptotic genes p53 decrease,(p<0.05); comparing the experimental group, BMSCs-MVs p53 gene expression compared with BMSCs-MVs group + inhibitor group EVP4593 reduction, P <0.05; BMSCs-MVs + reduce the amount of p53 gene expression inhibitor EVP4593 group compared with the inhibitor EVP4593, P <0.05; with the experimental group compared with each other at different time points(4h and 24h), p53 gene expression no significant difference in the amount, P> 0.05; 2.MVs group and the control group compared to inhibit apoptosis gene expression levels of Bcl-2 increased significantly, P <0.05; inhibitor EVP4593 group and BMSCs-MVs + inhibitor EVP4593 group were compared with the control group, the inhibition of apoptosis gene expression levels of Bcl-2 significantly reduced, P <0.05; BMSCs-MVs + EVP4593 inhibitor significantly reduced compared with the MVs group Bcl-2 gene expression, P <0.05; BMSCs-MVs + inhibitor group and inhibitor EVP4593 EVP4593 significantly reduced compared gene expression of Bcl-2, P <0.05; the same time in different experimental groups(4h and 24h) no significant difference compared with each other Bcl-2 gene expression, P> 0.05;Conclusions 1.BMSCs-MVs have reduced chondrocyte pro-apoptotic p53 gene transcription and increased cartilage cells inhibits apoptosis Bcl-2 gene transcription function; 2.BMSCs-MVs effect on chondrocytes through other NF-kb pathway signaling pathway other than to achieve, but the production process is involved in NF-kb pathway to that effect in a not yet completely clear.