Research Of Effect Oftopographies Of 316l On Biological Behavior Of Endothelial Cells And Smooth Muscle Cells

In this paper, by using the femtosecond laser processing technology, we constructed the topological structure of micro nano interface on 316 L stainless steel substrate. By using scanning electron microscopy and contact angle measurement to analysis and test on the samples, then we cultured human umbilical vein endothelial cells 、rat brain micro vascular endothelial cells and smooth muscle cells on these samples in vitro. By watching the influence of cell growth, aimed to find a interface which can regulate va scular endothelial cells’ and smooth muscle cells’ growth behavior of the micro nanostructure interface, which can provide a reference for the design of micro structure of the surface of stent materials.The human umbilical vein endothelial cells’ experience showed that the 316 L stainless steel had good cell compatibility; it was suitable for cells’ adhesion, growth and proliferation. The cells’ growth direction showed no clearly orientation and adhesion. The morphological index analysis experience showed that the topological structure can strengthen the regulation of the HUVECs orientation.The BMVECs-F5 experiments showed that the cells on the topological structure of the sample surface showed long fusiform shape, which the smooth surface’s cells were grew without obviously orientation. When cells were cultured for 3d, the number of the cells on topological structure’s surface was increased, cells spread into pieces on the micro structure of the stripe with the direction along the long fusiform. When at the same time the cells on the smooth surface of the sample were “whirlpool” distribution. Growth orientation analysis showed that the cells on micro structure region were found along the direction of fringe orientation which the cells on the smooth samples had no obvious orientation. The results could confirm that the micro topology interface was good for endothelial cells’ adhesion, spread and growth.The SMCs-F5 experiences showed that with the increase of culture time, SMCs had been adhered and spread on the sample surface, cells were arranged radically. Parts of the SMCs layers overlap, and some of the region showed monolayer cell layer of ups and downs, this was the smooth muscle cell characteristic--- "peak" and "Valley" growth pattern.After 3d for culture, cells adhesion of micro nano structure on the sur face of the sample was ahead of cell area on the polishing surface, which could be considered micro nano interface topology has a little inhibition effect spreading on SMCs.