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Study On Commercial Grade Specification And Rational Analysis Correlation Of Gansu Codonopsis Pilosula

Posted on:2017-05-13Degree:MasterType:Thesis
Country:ChinaCandidate:S B LiuFull Text:PDF
GTID:2284330503479149Subject:Pharmacy
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Objective: Taking the main planting counties area of Codonopsis producing has expanded in Gansu Province each year. The standard of traditional commercial specification has not objectively guide market of higher prices with better quality, which is impacting on the development of Codonopsis of Gansu planting industry seriously.The study use mainly Gansu different cultivation area’s Codonopsis pilosula(Baitiao Radix Codonopsis and Wen Radix Codonopsis) as the research object, In view of the real estate of Gansu Codonopsis planting status, uneven quality, the current commodity specification confusion for related research.To investigate the correlation between the evaluation index of commercial grade and quality of Radix Codonopsis, to put forward some suggestions of planting,which were reasonable,normative and scientific,and provide experience and methods for the standardization of industry.Methods: Through methods of literature review,expert consultation and field survey, we investigated species distribution,cultivate processing Codonopsis of Codonopsis produced from Gansu.Collected 30 different medicinal herbs codonopsis pilosula in main producing area,and measured of parameters appearance, Simultaneously, according to the 2010 edition of "Chinese Pharmacopoeia" method,to determinate the moisture, total ashes and alcohol-soluble extract contents. Lobetyolinand atractylenoide Ⅲ contents determined by HPLC,phenol-sulfuric acid chromogenic method is used to determine the Polysaccharide content,Using one-way ANOVA, factor analysis, Gray correlation method relational to comprehensive evaluate the data, analyze the relevance of commodity grade and quality evaluation.Results: 1 The Gansu merchandise codonopsis pilosula have mainly Baitiao Radix Codonopsis and Wen Radix Codonopsis. Baitiao Radix Codonopsis is mainly in Dingxi Prefecture,the cultivation area increased year by year; Wen Radix Codonopsis is mainly in Wen County and it’s surrounding counties,and were divided into multiple levels of commodity grade. In recent years, because of the technology level of planting is generally low in main place, marketing system management is disorder, lack of generally planning and regulation of local authorities, and many other factors, resulting in a phenomenon that a lower sales of cultivated codonopsis pilosula of value-added. Codonopsis planting area are significantly decline.2 In the powder Identification and cross-section, codonopsis pilosula from different place have some differences, but in different merchandise grades codonopsis,which is same origin,pilosula its character identification, microscopic identification insignificant.TLC has lobetyolin same spot color, but different shades of color, which showed that the Radix Codonopsis pilosula’s lobetyolin content vary in Gansu Province. The moisture are lower than the requirements of the codex, between 6.23 % ~ 8.10 %; The total ash content between2.25 % ~ 7.59 %, with an average content of 4.45 %, and 74.47% of the sample codonopsis pilosula qualified; lobetyolin content from different areas: Tanchang County’s Baitiao Radix Codonopsis> Minxian County> Longxi County> Weiyuan County> Zhangxian> Lintao County> Wudu> Wen County> Tanchang County’s Wen Radix Codonopsis; Different Places atractylenoideⅢ content: Tanchang County’s Baitiao Radix Codonopsis > Minxian County>Longxi County> Weiyuan County> Zhangxian> Lintao County> Wudu> Wen County>Tanchang County’s Wen Radix Codonopsis,and not detected atractylenoideⅢ ingredients on wild codonopsis pilosula; Polysaccharide content of different habitats: Tanchang County’s Wen Radix Codonopsis > Longxi County> Lintao County> Tanchang County’s Baitiao Radix Codonopsis > Lintao County Wudu> Wudu> Wen County> Weiyuan County> Minxian County> Zhang County. Wen Radix Codonopsis more than1.10 times to Baitiao’s.3Using SPSS 21.0 statistical software for PCR analysis, lobetyolin is the most significant factor of quality of codonopsis pilosula, It turn out that, Baitiao Radix Codonopsis is70 %,30 % Wen Radix Codonopsis by the rank of total score factor analysis in the top ten.explained generally quality of Baitiao Radix Codonopsis is better than Wen Radix Codonopsis’ quality;Ranked Grade Two compared with First grade, Grade third higher,It’s explained the current grade of codonopsis pilosula bit confusing in main producing areas. CA analysis can be grouped into four types. the sample(Baitiao Radix Codonopsis Lintao County, Baitiao Radix Codonopsis Weiyuan County, Wenxian Wen Radix Codonopsis’ and Wudu) clustered into Ⅰcategory, more than four place of production accounting for 79.07 % of the total. Samples(Baitiao Radix Codonopsis Tanchang County and the Min counties) clustered into Ⅱ category, samples(Baitiao Radix Codonopsis Zhang County, Longxi County, Wudu District Wen Radix Codonopsis) clustered into Ⅲ category,sample(Tanchang County wild grade First and the Second) clustered into Ⅳ category,. The results showed that the trade classification is not obvious, but the origin, species classification more obvious.4Based on Gray correlation analysis, it concluded that Baitiao Radix Codonopsis in Minxian,Lintao, Longxian accounted for 20% of total sample, Wenxian and the Wudu District accounted for 28.57 % and 68.75 % of the total samples of Wen Radix Codonopsis in the sample of relative relation> 0.465, which showed codonopsis pilosula are preferably quality above. Herbs ranking is not consistent with its merchandise grade, Grade Two relative to the origin of Grade One and Grade Three.Merchandise living ranking top 51, Baitiao Radix Codonopsis: Grade Two> Grade One > Grade Third; Wen Radix Codonopsis: Grade Two >Grade One > t Grade Third > Grade Four.Shijiba of Wen County even higher merchandise grain party level, after its ranking by more comprehensive quality worse situation.5 Codonopsis pilosula are compared for multi-index evaluation, origin factors(altitude,latitude and longitude) and lower herbs Arundo diameter, length, Moisture, Total ash,Polysaccharide was positively correlated with atractylenoide Ⅲ was significantly negative correlation; Merchandise grade and under herbs Arundo diameter was positively correlated,correlation coefficient being 0.551 and 0.218; Arundo under diameter by length with the moisture was a significant positive correlation, correlation coefficient were 0.779 and0.496,respectively; and lobetyolin and the atractylenoideⅢ were negative Related, but the correlation did not reach significant levels; Length atractylenoide contentⅢ negatively correlated.Conclusion:The resources and advantages of Gansu Codonopsis acreage does not translate into economic advantages, different cultivation regions, different grades of merchandise Gansu estate codonopsis pilosula quality and cultivation still need further study and utilization.It should be established in main producing areas codonopsis pilosula GAP Bases, stabilizing quality codonopsis pilosula, gradually transformed from rough to deep processing, the formation of high-quality, stable and efficient production patterns, to ensure that real estate Gansu Codonopsis pilosula produced sustained and healthy development. Factor total score and the gray evaluation results are supported in Weiyuan County, Lintao county as the core area of main producing areas of Baitiao Radix Codonopsis; Wenxian and Wudu area as the core area of Wen Radix Codonopsis Areas. At present, main producing areas codonopsis pilosula merchandise classification more confusion, the more merchandise grade level is not representative of the quality of the merits of medicines.Traditional commodity grading and quality evaluation has some relevance, but not correctly guide market transactions.External shape combined with the intrinsic quality indicators divided codonopsis pilosula Product rating method is more scientific and reliable.
Keywords/Search Tags:Codonopsis pilosula, Reasonableness, Gray correlation method, Merchandise grade, Relevance, Quality evaluation
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