Monodispire Dipeptide-based Nanocarrier Loading With Hydrophbic Photosensitive For In Vivo Photodynamic Therapy

The use of peptides as building blocks to create nanomaterials is a popular way in biomedical applications, such as drug delivery, tissue engineering and gene therapy.Among peptides molecules, dipeptide diphenylalaine(FF) and its derivates(such as cationic diphenylalanine, cysteine-diphenylalanine tripeptide) have attracted much attention due to their versatile properties, such as structural simplicity, ease of chemical modification, biocompatibility and especially strongly capability of assembly under different kinds of conditions. In this report, we used small aldehyde molecule to induce cationic diphenylalanine(CDP) to assemble into nanoparticles with uniform size.Noticeably, hydrophobic drug could easily load into the nanoparticles. By optimizing, the size of our nanocarriers could controlled winthin about 100 nm and zeta potential is about-30 mv that could let our nanoparticle concentrate in the tumor site by EPR effect.Photosensitizer Ce6 was loaded on our nanoparticles for photodynamic therapy.Efficient cell uptake by MCF-7 of Ce6-loaded nanocarriers was confirmed by confocal microscopy and in vitro cell cytotoxicity results showed that nanoparticles with Ce6 exhibit a much higher killing capacity of MCF-7 cells compared to the free Ce6.And in vivo anticancer therapy indicating that our nanoparticles have great potential application in drug delivery systems for cancer therapy.