The Effect Of Minimally Invasive Hematoma Aspiration On The JNK Signal Transduction Pathway

Objective:To explore the effect of minimally invasive hematoma aspiration(MIHA) on the JNK signal transduction pathway after intracerebral hemorrhage(ICH).Methods:In this experiment, 300 adult male Wistar rats were randomly and averagely divided into sham-operated group, ICH group and MIHA group. In each group, 60 rats were used in the detection of indexes in this experiment, while the other 40 rats were used to replace rats which reached the exclusion criteria(accidental death or operation failure). In ICH group and MIHA group, ICH was induced by injection of 70 μL of autologous arterial blood into rat brains, while only the rats in MIHA group were treated by MIHA 6 hours after ICH. Rats in sham-operated group were injected nothing into brains, and they weren’t treated either like rats in ICH group. In each group, six rats were randomly selected to observe their Bederson’s scales persistently(6 h, 24 h, 48 h, 72 h, 96 h, 120 h after ICH). According to the time they were sacrificed, the remaining rats in each group were divided into 3 subgroups(24 h, 72 h, 120 h). The change of brain water content(BWC) was measured by the wet weight to dry weight ratio method. The morphology of nerve cells in cortex was observed by the hematoxylin-eosin(HE) staining. The expressions of p JNK and JNK in perihematomal brain tissue were determined by the immunohistochemistry(IHC) and Western Blotting(WB).Results:At all time points, compared with the ICH groups, the expression of p JNK decreased obviously in MIHA groups(p<0.05), while the Bederson’s scales and BWC declined, and neuron injury in cortex relieved(p<0.05). The expression level of JNK was not altered at different groups. The data obtained by IHC and WB indicated a high-level of consistency, which provided a certain dependability of the test results.Conclusion:The JNK signal transduction pathway could be activated after intracerebral hemorrhage, with the expressions of p JNK increasing. MIHA could relieve the histopathological damage on nerve cells, reducing brain edema and neurological deficits, and these neuroprotective effects might be associated with supression of JNK signal transduction pathway.