Research H9 And HepG2 In Vitro Co-culture Supernatants Affect Hepg2 Proliferation, Migration And Apoptosis

Objective: To explore the influence of co-culture supernatant from embryonic stem cell H9 and hepatoma cell line Hep G2 on the proliferation, migration and apoptosis of hepatoma cell Hep G2.Methods:We conducted in virto co-culture of H9 and Hep G2, then obtained supernatants in 12 h, 24 h, 36 h and 48 h and let supernatants of different concentration, namely 20%, 40%, 60% and 80% to act on Hep G2 cells. We observed growth condition and morphological changes of the cells under an inverted fluorescence microscope. MTS method was employed to evaluate proliferation conditon of Hep G2 cells in 24 h and 48 h since supernatants of varied concentrations had been added. We applied flow cytometry to show the apoptosis of cells. We also detected apoptosis of cells using Hochest33258 chromosome. We probed influence of supernatants on migration of cells. We detect Hep G2 cells Expression profiles by Gene chip technique.Results: Co-culture supernatants of varied concentration all had inhibitive effect on proliferation of Hep G2 cells. Supernatants of concentrations higer than 40% promoted apoptosis, particularly early and late apoptosis of Hep G2 cells rather notably. Chamber method also revealed the supernatant’s inhibition to invasion and migration of Hep G2 cells, Simultaneously, Supernatant can also promote Hep G2 cell death,and Hep G2 cells Gene expression profiles and functional role of the supernatant have obvious differences.and the same time it had no conspicuous effects on normal hepatocyte LO2.Conclusions: Co-culture supernatant of embryonic stem cells and hepatoma cell Hep G2 significantly inhibits the proliferation, migration and invasion o hepatoma cell Hep G2 in virto while promoting its apoptosis. Its effectiveness is concentration-dependent and increases over time. These results prove the anti-tumor effectiveness of this supernatant.