The Study On The Effects Of Stemness Potential Of CD133- Cells Via X-ray Radiation In Nasopharyngeal Carcinoma

PART ONE The CD133- cells were sorted and the potential of stemness were identified in Nasopharyngeal CarcinomaObjective: To preliminary sort the CD133- cells in Nasopharyngeal Carcinoma and identify its stem-like potential.Methods: The CD133- cells were sorted by magnetics-activated cell sorting(MACS) in nasopharyngeal carcinoma, and the Flow cytometer(FCM) was employed to detect the purity of CD133+ and CD133- cells. And the stem-like characteristics were verified by invasion assay and tumor xenograft models in nude mice.Results: The purity of CD133+ and CD133- cells were 97.72%±0.82%and 1.63%±0.66%, respectively. And, compared with CD133- cells, the invasion and tumorigenesis had more significant in CD133+ cells.Conclusion: CD133- cells can be sorted by MACS, and it can be recognized as non-stem cells in nasopharyngeal carcinoma.PART TWO The stemness potential of CD133- cells were enhanced by X-ray radiation in nasopharyngeal carcinomaObjective: To investigate the role of X-ray radiation in induced the potential of CD133- cells transform to cancer stem cells in nasopharyngeal carcinoma.Methods: The CD133- cells were treated with 0、2、4、6、8Gy X-ray radiation, and the proliferation were detected by MTT assay. After the CD133- treated with the best dose of X-ray, the stem-like characteristics were verified by invasion assay and tumor xenograft models in nude mice.And RT-PCR and western-blot were performed to detect the expression of CD133, SOX2, OCT4 and Nanog.Results: The best radiation dose on CD133- is approximated 4Gy.After the CD133- treated with 4Gy of X-ray, the invasion and tumorigenesis were enhanced, and the expression of CD133, SOX2, OCT4 and Nanog were up-regulated.Conclusion: X-ray radiation can enhanced the stemness potential of CD133- cells by up-regulating the expression of stem-like genes, in nasopharyngeal carcinoma.