| Object: The present research focused on the drawbacks of curcumin and rutin on the low extraction rate from natural herb medicines and availability. To solve those problems, the microwave-assisted extraction has been going to be introduced into the extraction of curcumin and rutin in order to obtain higher extraction, and advanced nano materials were wished to be as available drug carriers with higher availability. Therefore, curcumin@nano-Mg(OH)2 and rutin@nano-Mg(OH)2 nano drug delivery systems were synthetized. Their efficiencies were evaluated by DPPH scavenging activity in vitro. Also, the antioxidant action of nano-drugs based on mice model was going to be estimated in this thesis.Methods: The curcumin from Curcuma longa L. and rutin from Sopho japomica were extracted by microwave-assisted extraction, detected by high performance liquid chromatography, and the extraction ratio was calculated. Then, the nano materials of nano-Mg(OH)2, curcumin@nano-Mg(OH)2 and rutin@nano-Mg(OH)2 were synthesized in step-by-step and characterized by TEM such as observation of appearance. The particle size was determined by Malvin particle size analyzer for determination of average radius and for the evaluation of their distribution. With Zeta potential measurement and FT-IR spectroscopy, the drug loading and its efficiency were identified and calculated. In addition, p H release efficiency of rutin@nano-Mg(OH)2 was examined. The scavenging activity was tested by DPPH method in vitro. Finally, this research has evaluated the antioxidant action of nano-drugs based on the acetaminophen-induced liver in mice.Results: 1. Obtained the operation conditions of extraction for curcumin: The microwave power is 400 W, extraction solvent was of 70% ethanol solution, the ratio to herb material to solvent was 1:30 and 1 minutes of extraction time. The extraction rate of curcumin was 4.62%. The extraction condition of rutin: The microwave power was about 400 W, extraction solvent was used double distilled water, the ratio of herb medicine to solvent was 1:100 and extraction time was 12 minutes. The extraction rate of rutin was 8.45%. 2. The shapes of Nano-Mg(OH)2 and curcumin@nano-Mg(OH)2 were round, while the shape of rutin@nano-Mg(OH)2 was irregular. The radius of nano particles of Nano-Mg(OH)2, curcumin@nano-Mg(OH)2 and rutin@nano-Mg(OH)2 were 70.89 nm, 85.87 nm and 132.2nm respectively. 3. The most high drug loading efficiencies of curcumin@nano-Mg(OH)2 and rutin@nano-Mg(OH)2 were 39.6% and 34.7% respectively. 4. In response to pH release rate experiment, rutin@nano-Mg(OH)2 release rate increased gradually, then reached in the platform after about 24 hours(under p H 7.4 and 5.0). Under p H 6.5, rutin@nano-Mg(OH)2 release rate was around 75%. 5. The DPPH scavenging activity of curcumin@nano-Mg(OH)2 and rutin@nano-Mg(OH)2 would increase with the concentration of durg or nanocarriers. 6. These samples could protect mice liver from damaging through decreasing ATL and AST activities and liver cells apoptosis. 7. Curcumin@nano-Mg(OH)2 and rutin@nano-Mg(OH)2 decreased MDA content, improved SOD activity and GSH level.Conclusion: This thesis has explored the extraction conditions of curcumin and rutin of microwave assisted extraction, and has succeed to construct the curcumin and rutin nano-drug carriers systems, and evaluated by mice model, which proved that these drugs protected against acetaminophen-induced liver injure in mice and nano-drugs played more effectively than traditional durgs. The Curcumin@nano-Mg(OH)2 and rutin@nano-Mg(OH)2 could much effectively scavenge DPPH, much easily drop down MDA and enhance SOD activity to maintain GSH level in cells, avoiding exhausting it, and thus decline oxidative stress. |
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