Changes Of Th17 Cells And CD4⁺CD25^highCD127^low T Cells Expression In Mice With Experimental Anti-phospholipid Syndrome Fed With Recombinant Human β2-glycoprotein 1

Objective:Antiphospholipid syndrome(APS) is an autoimmune disease which is caused by antiphospholipid antibodies(antiphospholipid antibodies, APA),especially during pregnancy can cause recurrent early abortion, pregnancy of unknown causes of intrauterine fetal death, fetal growth restriction, complications such as preeclampsia, and even affect the outcome of pregnancy.To observe the changes of T helper 17 cells( Th17) and CD4+CD25highCD127lowT cells in experiental anti-phospholipid syndrome(EAPS) mice fed with recombinant human β2-glycoprotein 1(rhβ2-GP1).Methods:1 、 EAPS model was established by immunizing BALB/c mice with rhβ2-GP1.Establishment of tolerance group by combined oral recombinant human 2-GP1 immune mice.2、Using flow cytometry detection method in the group, made up of the following groups: first, the control group; Second, the oral tolerance; Third, the model group. Percentages of CD4+IL-17+Th17 and CD4+CD25highCD127low T cells in peripheral blood mononuclear cells in mice from each groups were determined by flow cytometry.3、the expressions levels of ROR-γt m RNA and Foxp3 m RNA were detected by RT-PCR.Results:1、From the first month of immunization to fifth months,the percentages of CD4+IL-17+Th17 cells from PBMC in model group were higher than those of control group(p<0.05), the percentages of CD4+IL-17+Th17 cells in tolerized group were lower than model group, and paralleled to those of control group during oral tolerance induction(p>0.05).2、The expressing levels of ROR-γt m RNA in the model group were higher than those of control group(p<0.05), and the expressing levels in tolerized group were lower than those of model group(p<0.05)on the first、second、third、fourth and fifth months.3、From the first month of immunization to fifth months,the frequencies of CD4+CD25highCD127low T cells from PBMC in tolerized group were paralleled to those of control group during oral tolerance induction(p>0.05).4、The expressing levels of Foxp3 m RNA in the tolerized group were higher than those of control group(p<0.05)on the first、second、third months after immunization(p<0.05),but fourth months and there were no significant differences on fifth months between tolerized group and control group(p>0.05). The expressing levels of Foxp3 m RNA in the tolerized group were paralleled to model group on firstmonths(p>0.05)and the latter began to decrease after second months and were lower than those of control group significantly after fifth months(p<0.05).Conclusion:1、The expression level of Th17 cells in PBMC model group and the expression level of T in m RNA cells were increased compared with the control group,The mice in the tolerance group were similar to those of the control group by oral administration of recombinant human 2-GP1,Therefore, we believe that APS excessive differentiation progress of Th17 may have a certain relationship。2、The percentage of CD4+CD25highCD127low T cells in PBMC model group was decreased from third months to months,The percentage of CD4+CD25highCD127low T cells in the tolerance group was established by oral administration of recombinant human 2-GP1 in first months to fifth months. No differences were compared with the control group.Therefore, we think that oral tolerance may play a role in regulating the differentiation of T cells.