| ObjectiveTo analysis the risk factors and the changes of serum interleukin-10(IL-10), interleukin-18(IL-18) between both diseases in the primary gout with non-alcoholic fatty liver disease(NAFLD). Methods1. 40 healthy people were selected who were recruited in Tianjin. Selected 138 primary gout patients, who were admitted in Tianjin Medical University Metabolic Diseases Hospital during Oct. 2012 to Dec. 2014; 67 primary gout patients had diabetes mellitus. A total of 178 subjects including 156 men and 22 women were take part in this study.2. Records the following informations of the subjects:(1) Demographic data:sex, age, course,waist, hip, height, weight. WHR and body mass index(BMI) were calculated.(2) Laboratory parameters: platelet(PLT),hemoglobin A1c(Hb A1c), total cholesterol(CHO),triglyceride(TG), low-density lipoprotein(LDL-c), high-density lipoprotein(HDL-c), albumin(ALB), aspartate transaminase(AST),alanine aminotransferase(ALT), γ-gamma glutamyl transpeptidase(γ-GGT), fasting plasma glucose(FPG), fasting insulin(FINS),serum uric acid(SUA). Calculated insulin resistance index(HOMA-IR)=FPG × FINS/22.5 and insulin sensitivity index(ISI)=1/(FPG×FINS);(3) Measures human serum interleukin-18, interleukin-10 levels;(4) Acquisition liver B ultrasonic image, use the linear regression to calculate liver fat content.3. Grouping:(1) According to whether they had Gout, these subjects were divided into two groups: control group and gout group;(2) Divided gout group into four groups, according to the quartile of SUA levels. The four groups were as follows from low to high respectively: group A, group B, group C, group D;(3) Divided gout group into four groups, according to the quartile of liver fat content levels.The four groups were as follows from low to high respectively: group a, group b, group c, group d. Results1.Compared with control group, gout group showed a trend of increased detection rate of NAFLD(58% vs 35%)(P<0.05).2. Compared with control group, the BMI,WHR,SBP,DBP,TG,SUA,Hb A1 c,FINS,HOMA-IR,liver,fat content,IL-10,IL-18 were elevated obviously in gout group, HDL-c、ISI were descend obviously(P<0.05); The prevalence of obesity, hypertension,and hyperglycemia were significantly increased in gout group compared with control group(P<0.05).3. In the gout patients, as serum uric acid rise, BMI,SBP,DBP,ALT,AST,TG,FPG, FINS,HOMA-IR,IL-10,IL-18, liver fat content levels were elevated respectively, ISI were descend respectively(P<0.05).4. In the gout patients, as liver fat content rise, BMI,SBP,DBP, SUA,ALT,AST, FINS,HOMA-IR,IL-10,IL-18 were elevated respectively, ISI were descend respectively(P<0.05).5. Using the binary linear regression analysis, the results show SUA,HOMA-IR were the independent risk factors of NAFLD.6. Correlation analysis shows, liver fat content was positive correlation with SBP,DBP,BMI,ALT,AST,SUA,FINS,HOMA-IR,IL-10,IL-18,was negative correlation with ISI.7. Using the linear multivariate regression to analysis, liver fat content as the dependent variable, SBP, BMI,ALT,AST,SUA, HOMA-IR,IL-10,IL-18 as the independent variable,the results show SUA,IL-18 for regression equation of liver fat content(beta were 0.048,0.126,respectively)( P<0.05). Conclusions1. The primary gout patients had high prevalence of NAFLD. The liver fat content elevated obviously and elevated with serum uric acid levels increasing.2. Insulin resistance, obesity, hypertension and lipid metabolism disorder were the important risk factors for the primary gout patients developing NAFLD.3. Interleukin-18 and interleukin-10 may play an important role in primary gout patients with NAFLD. |
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