| Objective To study radiation protectionmechanism of Glycyrrhizin(GL). Pharmacokinetic of Glycyrrhetic Acid(GA) after ig. GL and Bu-Zhong-Yi-Qi-Wan(BY) or in normal and radiation injured mice were compared.Methods MSC of C57BL/6 mice were cultured and injured by 12 Gy 60 Co γ irradiation; The cytotoxicity of GL were tested by CCK-8; the effect of GL and GA on SOD activity in MSC were studied; the level of candidate genes were tested by RT-PCR; expression of Tnfaip-2 were tested by Western blot.Rats or mices were ig.GLand BY;Normal and radiation injured micewere ip. or ig. GL; Plasma and bloodsamples were extracted with liquid-liquid extraction or proteins precipition, separated by liquid chromatography on a C8 reversion phase chromatographic column. Concentration of GAwasdetected by the LC-MS/MS. Pharmacokinetic profiles wereconstructed by Das 2.0 software, andthe pharmacokinetic parameterswerecomparedby SPSS 16.0.Results The SOD activity were significantly incresed after treated by GL and GA. GL and GA could enhance theexpression of Tnfaip-2in MSC after radiation; GL could increase the proteinproduction of Tnfaip-2.BY reduced the Cmax and AUC0-t of GA by 56% and 76%, respectively. After ip. GL, AUC0–t and Cmax of GL in radiation injured mice were reduced 24.5% and 46.1% and the Tmax of GA take 5 hours difference; After ig. GL, AUC0–t and Cmax of GA in radiation injured mice are reduced 29.6% and 34.2%.Conclusion GL mayhave radioprotection against damage in MSC by increase the SOD activity and change the expression of Tnfaip-2.the results indicated that some components in the BY had aninhibition effect on the absorption process of Glycyrrhizin in the gut. The influence of radiation to intestinal may change the way of absorption and metabolism. |
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