Effect Of Icaritin On The Proloferation And Intracellular Reactive Oxygen Species In K562 Cells

Objective: The aim of this study was to investigate the effect of Icaritin(ICT) on the proliferation and Reactive oxygen species(ROS) of K562 cells.Methods: The effect of K562 cell proliferation was detected by MTT assay, Cell apoptosis and Intracellular Reactive Oxygen Species was detected by Flow Cytometry, The expression of PARP were measured with Western Blot.Results: 1.The inhibitory rates on proliferation of K562 cells treated by different conce- ntrations of ICT for 48 h is significantly increased.(0),(6.3 ± 1.48)%,(25 ± 1.86%),(32 ± 3.07%),(66 ± 2.07%),(76 ± 1.68%)and(80 ± 3.69%),which changes dependent on a concentration(r = 0.837), ICT treated in K562 cells 48 hours the IC50(half inhibitory concentration) was 12.41 μmol / L. 2. The apoptosis rate of K562 cells treated by different concentrations of ICT for 48 h is significantly increased.(0.6 ± 0.38%),(0.66 ± 0.41%),(6.36 ± 1.56%),(23.01 ± 0.59%),(30.24 ± 2.11%),(36.01 ± 1.69%),and in a dose effect relationship(r=0.904). 3. The expression of PARP89 KD of K562 cells treated by different concentrations of ICT for 48 h is significantly increased.With the increase of the concentration of icaritin, increased expression of PARP. 4. The ROS mean fluorescence intensity percentage of K562 cells treated by different concentrations of ICT for 48 h is gradually increased(74.34 ± 2.08,84.86 ± 1.46,90.14 ± 3.37,88.82 ± 7.38,88.4 ± 1.87)(r = 0.60).Conclusion: The effects of on the proliferation and apoptosis of K562 cells were inhibited, and the mechanism was related to the increase of reactive oxygen species in the cells.