Biological Characteristics Of CD133~+CD44~+ Cancer Stem Cells Sorting From Laryngeal Carcinoma Cell Line TU177

Objective :Magnetic activated cell sorting was used to separate CD133+CD44+ cancer cells from laryngeal carcinoma TU177 cell line. Analysis the biological characteristics of these subpopulations.Methods :After culture,TU177 cells were subjected to magnetic activated cell sorting to obtain CD133+CD44+、CD133+CD44-、CD133-CD44+、CD133-CD44- cells. Evaluate the efficiency of magnetic separation by flow cytometry. Test cell proliferation,migration, invasion, adhesion, colony forming ability of the cells,Suspension culture to detect the ball forming ability.Results:CD133+CD44+ cells show higher proliferation, migration, invasion, adhesion,clone ability than other groups(P<0.0001). The proliferation, migration, invasion and colony forming tests show that there were no differences between TU177 and CD133-CD44- cells(P>0.05), well other groups show statistic difference from each other.(P<0.0001).There are no difference between the TU177 and CD133-CD44+groups in the adhesion experiment( P=0.635), other groups show significant difference from each other(P<0.0001).CD133+CD44+ cells in serum-free medium can obviously form the balloon.Conclusions:TU177 cells can be serparated by Magnetic activated cell sorting effectively.CD133+CD44+ cells have higher proliferation, invasion, migration, adhesion,clone forming ability than others.These cells shows stem cell-like properties.As stem cell marker, CD133 is more powerful than CD44.Our study may provide evidence for target treatment of laryngeal cancer.