The Study Of Atorvastatin Calcium-nanostructured Lipid Carriers

Atorvastatin Calcium(ATRS),an inhibitor of 3-hydroxy-3-methyglutarylcoenzyme A(HMG-Co A) reductase, works fast and lowers lipid heavily. However,because of its low water solubility and fitst-pass elilmination, the oral bioavailability of ATRS is quite low. The objective of this study was to prepare a kind of Atorvastatin Calcium-nanostructured lipid carriers(ATRS-NLC), inorder to promote its absorption and improve its oral bioavailability.A high performance liquid chromatography(HPLC) method of ATRS was developed to determine its concentration in vitro. Inaddition, the HPLC method to content ATRS in plasma was evaluated, of which specific property and linear was quite appropriate.On the base of single factor in vestigation,CCD-Behnken experimental design was performed to work out the optimal prescription.The microscopic morphology was nearly spherical.Its diameter was95.7±12.8nm with Zeta potential being-13.72 m V,and the entrapment efficiencyis 71.5%.The freeze-drying method was used to make ATRS-NLC into solid, which was good-looking and appropriate diameter.The ATRS-NLC sample was prepared with the optimal formulation. The pharmacokinetic study was performed in Sprague-Dawleyrats,with there ference of ATRS-Suspension. The Cmax of the ATRS-NLC was 2.3 times us much us which of ATRS-Suspension. The AUC of ATRS-NLC was 4.1 times higher than which ofATRS-Suspension. There results showed that ATRS-NLC could enhance the oral bioavailability of ATRS.