Effect Of Hypoxia Microenvironment On Growth Of Glioma U251 Cells In Vitro

Objective: Take U251, the common human glioma cell line as the research object. To investigate the effect of hypoxia microenvironment on growth, proliferation, cell cycle distribution and apoptosis of human glioma U251 cells in vitro.Methods: The experiment was carried out as follows: the U251 cells were cultured under different concentrations of oxygen(normoxia, 3% hypoxia, and 1% hypoxia) in three groups. Then the cell morphology was observed by inverted phase contrast microscope. The cell proliferation was measured by CCK-8 assay. The clony forming ability was measured by plate clone formation test. The cell cycle distribution and apoptosis were determined by FCM method. Materials were analyzed by SPSS 22.0 software for data correlation analysis, as to evaluate the relevant indicators of hypoxia.Results: The U251 cells in 3% hypoxia group grew better than those in normoxia group and 1% hypoxia group. The proliferation activity and clony forming ability of U251 cells in 3% hypoxia group were significantly better than those in normoxia group, while those in normoxia group was better than those in 1% hypoxia group(P < 0.05 in all cases). The ratio of proliferating cells(at S phase and G2 phase) in U251 cells of 3% hypoxia group was higher than that in normoxia group, while that of normoxia group was higher than that of 1% hypoxia group(P < 0.05 in all cases). The apoptosis rate of U251 cells in 3% hypoxia group was lower than that in normoxia group, while that in normoxia group was lower than that in 1% hypoxia group(P < 0.05 in all cases).Conclusion: From cell growth, proliferation, cell cycle distribution and apoptosis of U251 cells in vitro, the findings of the experiment show the conclusions below. Moderate hypoxia microenvironment(3%) has the potential to promote the growth and proliferation of glioma U251 cells, and to increase the cell proportion at the proliferating phase, as well as to inhibit the process of apoptosis in vitro.