Co-regulation Of KSP And CDH1 Gene Expression Effectively Inhibits The Proliferation Of HepG2 Cells

Objective: This paper discussed the expression of down-regulating kinesin spindle protein gene(KSP) and up-regulating CDH1 gene, and research on the effects of invasion and proliferation in A549 lung cancer cell.Methods: With transfetion reagent, the si RN A of targeting KSP gene and the sa RNA of targeting CDH1 gene promoter were transfected into A549 cells. According to the differences of transfection mixture, A549 cells could be divided into five groups, respectively, A group, B group, C groups, D groups and E groups. In the study, we detect cell proliferation ability was by MTT assay; The transwell invasion assay was used to detect the cell invasion; the activity of KSP and C DH1 gene m RN A were measured by real-time quantitative PCR; The expression of KSP, E-cadherin were detected by western blot.Results: After si RNA and sa RNA treatment of A549 cells, the cell proliferation was significantly decreased(p<0.05), and the number of A549 cells through the basement membrane was significantly reduced(p<0.05). The results of fluorescence quantitative PCR showed that si RNA significantly reduced the expression of KSP gene(p<0.05), and the sa RN A was significantly upregulated CDH1 gene(p<0.05). Western blot results showed that the expression of KSP was significantly reduced(p<0.05); the expression of E-cadherin was significantly increased(p<0.05). And it obviously showed that the proliferation and invasion of the co-transfected cell were better inhibited than si RNA or sa RNA transfected cell(p<0.05).Conclusion: The expression of KSP gene can be effectively downregulated by si RNA, and the expression of CDH1 gene can be upregulated by sa RNA. The invasion and proliferation of A549 cell were decreased, the mechanism may be related to the inhibition of epithelial- mesenchymal transformation, and it possibly may enhance cells apoptosis by some signaling pathway. The regulation effect of si RNA and sa RNA combined application is better than that of a single gene.