An Experimental Study Of A PLGA/nHA Scaffold BMP-2 Sustain-release System Composite Manufactured By 3D-bioprint Technique

PURPOSE:The polylactic-co-glycolic acid/nano-hydroxyapatite(PLGA/n HA) scaffold-bone morphogenetic protein-2(BMP-2)bionic tissue engineering bone composite was fabricated by 3D-Bioprinter.To test the mechanical character, the volume and quality degradation and the sustained-release performance of BMP-2 then evaluate the feasibility as tissue engineering bone.METHODS:BMP-2 chitosan nanosphere prepared by the ionic crosslinking method were loaded on temperature sensitive chitosan hydrogel made by chitosan andβ- glycerol phosphate to forming the cytokine release system. PLGA/n HA scaffold-BMP-2 sustained-release composite fabricated by 3D-bioprinting instrument and then the biological properties was tesetd by scanning electron microscope(SEM)and universal tester. The SPSS22.0 statistical software for statistical analysis.RESULTS:The morphology of PLGA/n HA scaffold fabricated by 3D-Bioprinter is well formed with inter-connected pore and regular pore shape. The pore size of PLGA/n HA scaffold is(431.31±18.40)μm and the porosity is(73.64±1.82)%;The compressive strength is(5.86±0.43)MPa; In 16 weeks observation, the quality degradation is(45.58±2.33)%, the original volume hold proportion is(45.3±1.81)%,the p H value is(5.53±0.08). The accumulative release of BMP-2 of PLGA/n HA composite is(7.68±0.26)% in first 24 h and reached(61.38±2.39)% at the 30 th days and still followed a promising sustained-release performance.CONCLUSIONS:The tissue engineering bone composite fabricated by 3D-Bioprinting technique shows a uniformed morphology with well mechanical property and stable release performance. The degradation rates and the p H value which stayed in an acceptable status have less effect on BMP-2 could meet the regeneration rate of new bone and the requirement of tissue engineering bone.