The Relevance Between NF-κB, MAPK And Akt Signaling Pathways And Proliferation Of Human Esophageal Cancer Cell Line Expressing P75^NTR In Nuclear

Objective: To study the relevance between NF-κB, MAPK and Akt signaling pathway and the proliferation of p75 NTR nuclear-positive cells derived from human esophageal cancer cell line Eca109.Methods: Eca109 cells were cultivated in serum-free culture medium to get minisphere, and selecting the p75 NTR nuclear-positive cell.Immunohistochemical method and western blot were used to test the protein expression and phosphorylation of p65,p38,JNK,ERK,Aktin p75 NTR nuclear-positive cells, respectively.Results: Immunohistochemical results showed that the protein expression and phosphorylation of p65,p38,JNK, ERK,Akt were all observed in p75 NTR nuclear-positive and p75 NTR cytoplasm-positive cells, respectively.Western blot experiments showed that, comparing with p75NTRcytoplasm-positive cells, the protein expression and phosphorylation ofp38 in p75 NTR nuclear-positive cells had a higher levels, and others protein expression and phosphorylation were lower.Conclusions: p38/MAPK signaling pathway was activated in p75NTRnuclear-positive cells, and NF-κB, JNK/MAPK, ERK/MAPK and Aktsignaling pathway was inhibited.