| Objective: To explore the relationship of the ADAM33 gene Q-1,T2 single nucleotide polymorphism(SNP) and suffering from chronic obstructive pulmonary disease(COPD) in Xinjiang Kazakh and Han population. Methods: Adopt Case-control study.1.Peripheral blood samples from cases group 197 cases and control group 193 of Kazakh, cases group 199 cases and control group 210 cases of Han were collected in Xinjiang area to extract DNA.2.The single nucleotide polymorphisms of Q-1 and T2 in ADAM33 gene were detected by SNa Pshot SNP genotypes and alleles. 3.Using SPSS20.0 statistical software for statistical analysis of all data, statistical analysises adopt χ2 test,One-way ANOVA, F test, Logistic regression,the values of odds ratio and 95% confidence intervals for odds ratio. Results: 1.The results of the comparison of basic information on two national : gender(male/female), age(years), smoking index(number of cigarettes smoked Per day×years of smoking, cigarettes/year)the differences were not statistically significant(P> 0.05); Lung function indices FEV1 predicted value(%), FEV1/FVC compared, the differences were statistically significant(P <0.05). 2.Two national COPD grou P and healthy control group ADAM33 gene Q-1, T2 locus genotype distribution in line with the Hardy-Weinberg genetic equilibrium law(P> 0.05).3. On Kazakh the ADAM33 gene Q-1 locus genotype constitute than GG,GA,AA were 78.2%,17.3%,4.6%;T2 locus GG,GA,AA genotype were81.7%,17.3%,1.0% of COPD group; genotype constitute than Q-1 locus GG,GA,AA genotypes were : 81.8%,17.7%,0.5%; T2 locus GG,GA,AA genotype were : 80.8%,18.7%,0.5% of control grou P. Com Parison of ADAM33 gene Q-1 locus genoty Pe frequencies distribution difference were statistically significance(Q-1:χ2=6.366,P=0.042),but T2 locus is not(T2:χ2=0.428,P=0.807). On Han the ADAM33 gene Q-1 locus genotype constitute than GG,GA,AA were 82.9%,16.6%,0.5%;T2 locus GG,GA,AA genotype were 77.3%,22.2%,0.5% of COPD group; genotype constitute than Q-1 locus GG,GA,AA genotypes were : 75.0%,24.5%,0.5%; T2 locus GG,GA,AA genotype were : 80.0%,19.0%,1.0% of control group. Comparison of ADAM33 gene Q-1,T2 locus genotype frequencies distribution difference were not statistically significance(Q-1:χ2 =3.9 12,P=0.141; T2:χ2=0.873,P=0.646). 4. On Kazakh the ADAM33 gene Q-1 locus allele frequencies constitute than of COPD group G,A allele were: 86.8%,13.2%; T2 locus G,A allele were: 90.4%,9.6%; Q-1 locus allele frequencies constitute than of control group G,A allele were: 90.6%,9.4%; T2 locus G,A allele were: 90.2%,9.8%.Comparison of ADAM33 gene Q-1, T2 locus allele frequencies distribution difference were not statistically significance(Q-1: χ2=2.833,P=0.092;T2:χ2=0.009,P=0.925).On Han the ADAM33 gene Q-1 locus allele frequencies constitute than of COPD group G,A allele were: 91.2%,8.8%; T2 locus G,A allele were: 88.4%,11.6%; Q-1 locus allele frequencies constitute than of control group G,A allele were: 87.3%,12.7%; T2 locus G,A allele were: 89.5%,10.5%.Comparison of ADAM33 gene Q-1, T2 locus allele frequencies distribution difference were not statistically significance(Q-1:χ2=3.285, P=0.070; T2:χ2=0.270,P=0.603).5.The relationship between the Q-1 locus genotype and lung function in the case group: compare with Q-1 locus and FEV1/FVC difference were statistically significance on Kazakh and Han(Kazakh Q-1:F=3.74 5,P=0.025;Han Q-1:F=3.831,P=0.0230),but FEV1% predicted was not(Kazakh Q-1:F=0.713,P=0.491;Han Q-1:F=1.881,P=0.155).6. Compare Kazakh AA genotype,Han GA genotype with GG genotype difference were statistically significance(Kazakh Q-1:P=0.036,OR= 9.175,95% CI= 1.149-73.290; Han Q-1:P=0.049,OR=0.612,95%CI=0.375-0. 998).Conclusion: 1. ADAM33 Q-1 locus polymorphism may relationship with COPD susceptibility in Xinjiang Kazakh, but T2 locus is not. ADAM33 Q-1,T2 locus polymorphism may no relationship with COPD susceptibility in Han population. 2.There is a realationship between ADAM33 gene Q-1 locus and FEV1/FVC decrease. |
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