Effect Of RNA Interference-related GPC3 Gene Expression On Lung Adenocarcinoma In Vitro

Objective: To investigate the changes of the biological behavior of human lung adenocarcinoma A549 cells after the GPC3 gene being interferenced by siRNA, to provide the theoretical foundation for early diagnosis, predict prognosis and treatment of human lung adenocarcinoma.Materials and methods: A549 cells were cultured in medium without antibiotics, after 24 h, Lipofectamine 2000 and si RNA were added into Opti-MEM separately, and mixed at room temperature for 5 min. Then the the two kinds of mixture were mixed at room temperature for 20 min. Last, mixture was added into the A549 cells. Cells were maintained at 37 °C in 5 % CO2 for 24 h. Then the cells in six-well were divided into three groups, each was called the blank group, the NC group and the interference group. The expression level of GPC3 was detected by real-time PCR and western blot. Cell proliferation was determined by CCK-8; apoptosis was measured by flow cytometry; migration was measured by scratch test.Results: After being interfered by siRNA, the expression of GPC3 gene was significantly decreased(P<0.05), both of the proliferation and migration ability of A549 cells reduced, but, the apoptosis rate was significantly increased(P<0.01), and there had no significant difference between GPC3-siRNA-NC and blank group.Conclusions: The expression of GPC3 gene in A549 cells was successfully interferenced by gene transfection technology which mediated by Lipofectamine 2000. The effect of GPC3 gene expression on lung adenocarcinoma in vitro was discussed preliminarily, and our results sugest that inhibit the expression of GPC3 gene can inhibit the proliferation and migration ability of lung adenoca- rcinoma A549 cells, but promote its apoptosis.