NGR Peptide-modified Pegylated Quantum Dots Crossing The Blood-brain Barrier And Targeted Fluorescence Labeling Of Glioma And Tumor Vasculature In Vitro

Objective To investigate the way Of NGR Peptide-modified PEGylated Quantum Dots For Crossing The Blood-Brain Barrier and the mechanism of Targeted Fluorescence labling Of Glioma And Tumor Vasculature.Methods Establishment of BBB and BTB models in vitro(1) Primary culture of rat Brian capillary endothelial cells(BCECs) and astrocytes;Identification of rat BCECs and astrocytes by immunostaining; Identify cell purity by flow cytometry.(2) the efficiency of BBB and BTB models tested by measuring the TEER values and permeability of sodium fluorescein.2.the characterization of NGR-PEG-QDs.(1) the diameter of NGR-PEG-QDs was measured by Zetasizer Nano ZS particle size analyzer;The stabilities of PEG-QDs in different pH was measured by fluorescence analyzer.(2)the proliferation of various cells after incubation with NGR-PEG-QDs were tested by CCK-8 method.3.(1)The Penetration rates of NGR-PEG-QDs across the BBB and BTB were measured byfluorescence plate reader( 2) The green fluorescent expression of NGR-PEG-QDs in Glioma And Tumor Vasculature were dected by confocal laser-scanning microscopy.Results The purity of rats Brian capillary endothelial cells and astrocytes cells were above 90%. the BBB and BTB models functioned well.2.The sizes of NGR-PEG-QDs were less than 100 nm;The fluorescence intensity of NGR-PEG-QDs in different pH conditions was no significant differences; No remarkable cytotoxicity of NGR-PEG-QDs to cells.3.the NGR-PEG-QDs could cross the BBB and BTB models and the green fluorescent could be detected by confocal laser-scanning microscopy.Conclusion The NGR-PEG-QDs can Targeted Fluorescence labling in Glioma And Tumor Vasculature in vitro by combining CD13.