Role Of Lin28A/B Subtypes In Apoptosis Of Hepatocellular Carcinoma Cells

Objective: To explore the role of Lin28A/B subtypes in apoptosis of hepatocellular carcinoma(HCC) cells, and to analyze the mechanism of action of Lin28A/B in tumorigenesis of HCC, determine the potential for clinical liver cancer therapeutic targets provide a theoretical basis.Method: The mRNA and protein expres- sion of Lin28A/B in HCC cells was detected by real-time PCR and Western blot. After si RNAs targeting Lin28A/B were transfected into HCC cells, the expression of Lin28A/B was detected by real-time PCR and Western blot, cell pro-liferation was assessed by MTT assay, and cell apoptosis was analyzed by FACS.Results:1. Real-time PCR showed that the ex- pression of Lin28A/B m RNAs was up-regulated in HCC cells(P < 0.05; P < 0.01), and the up- regulation of Lin28 B was more significant than that of Lin28 A.2. Transfection of Lin28A/B si RNAs decreased the expression of Lin28A/B in HCC cells(P < 0.05).3. MTT assay revealed that Lin28 A and Lin28 B si RNAs significantly inhibited HCC cell proliferation(P < 0.05; P < 0.01) and promot- ed cell apoptosis(P < 0.05; P < 0.01).4. The effect of Lin28 B si RNA on cell proliferation and apop- tosis was stronger than that of Lin28 A si RNA(P < 0.05).Conclusions: Both Lin28A/B take part in the tumorigenesis of HCC.Lin28 B may play a major role in HCC tumorigenesis, while Lin28 A may play a subordinate role.