Study On Antiproliferative Effect And Mechanism Of Ampelopsin Sodium On Human Lung Adenocarcinoma Cell Line SPC-A-1

Objective:The present study aimed at investigating the cytotoxic effect and the molecular mechanism of ampelopsin sodium (AMP-Na) on Human Lung Adenocarcinoma Cell SPC-A-1 alone or combined with carboplatin.Methods:The tumor cells were divided in groups treated with AMP-Na alone or combined with carboplatin and MTT colorimetric assay was employed to detect the antiproliferative effect. The changes of the expression of cell cycle related factors cyclin E、cyclin D1、CDK2、P53 and P21 were examined by Western Blotting.Results:1. AMP-Na alone decreased SPC-A-1 cells survival rate in a concentration-dependent manner ranging from 6.25-200μg/ml after 48 hours.2. The inhibitory effect of AMP-Na combined with carboplatin was greater than each one used alone, and IC50 obviously reduced.3. Western blot analysis showed that AMP-Na100μg/ml significantly decreased the expression of cyclinE and cyclin-dependent kinase CDK2, and up-regulated the expression of P21. AMP-Na 50μg/ml combined with carboplatin 25μg/ml significantly decreased the expression of CDK2、P53, and elevated the expression of P21. But AMP-Na showed no obvious interference with cyclin D1. Conclusion:1. AMP-Na decreases SPC-A-1 cells survival rate in a concentration-dependent manner, and showed significant cytotoxic activity.2. The antitumor activity of carboplatin was enhanced when combined with AMP-Na, the dosage of carboplatin was decreased and so was the toxicity.3. AMP-Na could significantly inhibit the growth of SPC-A-1 cells and arrest SPC-A-1 cells in G1 phase by decreasing the expression of cyclin E and cyclin-dependent kinase CDK2, and up-regulated the expression of P21 and P53.